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Literature summary extracted from

  • Yuan, J.; Sheppard, K.; Soell, D.
    Amino acid modifications on tRNA (2008), Acta Biochim. Biophys. Sin. (Shanghai), 40, 539-553.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.73
-
 Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.73 O-phospho-L-seryl-tRNACys + sulfide Methanococcus maripaludis the sulfur donor for this enzyme is unknown though in vitro sulfide is sufficient. Methanococcus maripaludis encodes both the direct and indirect paths for Cys-tRNACys synthesis. While sepS (encoding SepRS) can be deleted when the organism is grown in the presence of Cys, pscS (encoding SepCysS) cannot. SepCysS may possess an additional function in Methanococcus maripaludis that is essential L-cysteinyl-tRNACys + phosphate
-
 ?
6.1.1.27 ATP + O-phospho-L-serine + tRNACys Methanococcus maripaludis Methanococcus maripaludis encodes both the direct and indirect paths for Cys-tRNACys synthesis. SepS (encoding SepRS) can be deleted when the organism is grown in the presence of Cys AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.73 Archaeoglobus fulgidus
-
-
-
2.5.1.73 Archaeoglobus fulgidus O30207
-
-
2.5.1.73 Methanococcus maripaludis
-
-
-
2.5.1.73 no activity in Methanobrevibacter smithii
-
-
-
2.5.1.73 no activity in Methanosphaera stadtmanae
-
-
-
6.1.1.27 Methanococcus maripaludis
-
-
-
6.1.1.27 no activity in Methanobrevibacter smithii
-
-
-
6.1.1.27 no activity in Methanosphaera stadtmanae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.73 O-phospho-L-seryl-tRNACys + sulfide
-
 Archaeoglobus fulgidus L-cysteinyl-tRNACys + phosphate
-
 ?
2.5.1.73 O-phospho-L-seryl-tRNACys + sulfide the sulfur donor for this enzyme is unknown though in vitro sulfide is sufficient. Methanococcus maripaludis encodes both the direct and indirect paths for Cys-tRNACys synthesis. While sepS (encoding SepRS) can be deleted when the organism is grown in the presence of Cys, pscS (encoding SepCysS) cannot. SepCysS may possess an additional function in Methanococcus maripaludis that is essential Methanococcus maripaludis L-cysteinyl-tRNACys + phosphate
-
 ?
2.5.1.73 O-phospho-L-seryl-tRNACys + sulfide the sulfur donor for this enzyme is unknown though in vitro sulfide is sufficient Archaeoglobus fulgidus L-cysteinyl-tRNACys + phosphate
-
 ?
2.5.1.73 O-phospho-L-seryl-tRNACys + sulfide the sulfur donor for this enzyme is unknown though in vitro sulfide is sufficient Methanococcus maripaludis L-cysteinyl-tRNACys + phosphate
-
 ?
6.1.1.27 ATP + O-phospho-L-serine + tRNACys
-
 Methanococcus maripaludis AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?
6.1.1.27 ATP + O-phospho-L-serine + tRNACys Methanococcus maripaludis encodes both the direct and indirect paths for Cys-tRNACys synthesis. SepS (encoding SepRS) can be deleted when the organism is grown in the presence of Cys Methanococcus maripaludis AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.73 dimer x-ray crystallography Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.73 Sep-tRNA:Cys-tRNA synthase
-
 Archaeoglobus fulgidus
2.5.1.73 Sep-tRNA:Cys-tRNA synthase
-
 Methanococcus maripaludis
2.5.1.73 SepCysS
-
 Archaeoglobus fulgidus
2.5.1.73 SepCysS
-
 Methanococcus maripaludis
6.1.1.27 SepRS
-
 Methanococcus maripaludis

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.73 pyridoxal 5'-phosphate
-
 Archaeoglobus fulgidus
2.5.1.73 pyridoxal 5'-phosphate
-
 Methanococcus maripaludis
2.5.1.73 pyridoxal 5'-phosphate dependent Archaeoglobus fulgidus