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Literature summary extracted from

  • Mortazavi, M.; Hosseinkhani, S.; Khajeh, K.; Ranjbar, B.; Emamzadeh, A.R.
    Spectroscopic and functional characterization of Lampyris turkestanicus luciferase: a comparative study (2008), Acta Biochim. Biophys. Sin. (Shanghai), 40, 365-374.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.7 expressed in Escherichia coli strains XL1-Blue and BL21 Photinus pyralis
1.13.12.7 expressed in Escherichia coli strains XL1-Blue and BL21 Lampyris turkestanicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.7 0.02
-
 D-luciferin at pH 7.8, in 50 mM Tricine-NaOH buffer with 10 mM MgSO4 Lampyris turkestanicus
1.13.12.7 0.03
-
 D-luciferin at pH 7.8, in 50 mM Tricine-NaOH buffer with 10 mM MgSO4 Photinus pyralis
1.13.12.7 0.0955
-
 ATP in 50 mM Tricine-NaOH buffer with 10 mM MgSO4, at pH 7.8 Lampyris turkestanicus
1.13.12.7 0.14
-
 ATP at pH 7.8, in 50 mM Tricine-NaOH buffer with 10 mM MgSO4 Photinus pyralis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.12.7 Mg2+ the enzyme catalyzes the oxidation of substrate luciferin in the presence of Mg2+ (10 mM) Photinus pyralis
1.13.12.7 Mg2+ the enzyme catalyzes the oxidation of substrate luciferin in the presence of Mg2+ (10 mM) Lampyris turkestanicus

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Lampyris turkestanicus Q5UFR2
-
-
1.13.12.7 Photinus pyralis Q27758
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.7 Ni-NTA Sepharose column chromatography Photinus pyralis
1.13.12.7 Ni-NTA Sepharose column chromatography Lampyris turkestanicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 D-luciferin + O2 + ATP
-
 Photinus pyralis oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?
1.13.12.7 D-luciferin + O2 + ATP
-
 Lampyris turkestanicus oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.7 firefly luciferase
-
 Photinus pyralis
1.13.12.7 firefly luciferase
-
 Lampyris turkestanicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.7 25
-
-
 Photinus pyralis
1.13.12.7 25
-
-
 Lampyris turkestanicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.12.7 25 45 activity starts to decrease above 25°C and is almost completely lost at 45°C Photinus pyralis
1.13.12.7 25 45 activity starts to decrease above 25°C and is almost completely lost at 45°C Lampyris turkestanicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.7 8
-
-
 Photinus pyralis
1.13.12.7 8.5
-
-
 Lampyris turkestanicus