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Literature summary extracted from

  • Zhang, S.T.; Shi, J.; Zhao, J.; Qi, Y.F.; Guo, A.G.
    Expression of soluble and functional snake venom fibrinolytic enzyme fibrolase via the co-expression of DsbC in Escherichia coli (2006), Protein Pept. Lett., 13, 559-563.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.72 co-expression of the active signal-less His-tagged enzyme with chaperone DsbC in Escherichia coli cytoplasm, over 90% of the recombinant enzyme is soluble due to DsbC, while the chaperones FkpA and Skp do not have an effect on fibrolase solubility Agkistrodon contortrix contortrix

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.72 extracellular
-
 Agkistrodon contortrix contortrix
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.72 Zn2+ a zinc metalloprotease Agkistrodon contortrix contortrix

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.72 Fibrin + H2O Agkistrodon contortrix contortrix degradation of fibrin clots ?
-
 ?
3.4.24.72 fibrinogen + H2O Agkistrodon contortrix contortrix bovine substrate fibrin + propeptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.72 Agkistrodon contortrix contortrix P28891 southern copperhead snake
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.72 recombinant soluble His-tagged enzyme from Escherichia coli by nickel affinity and hydrophobic interaction chromatography to 95.6% purity Agkistrodon contortrix contortrix

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.72 venom
-
 Agkistrodon contortrix contortrix
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.72 Fibrin + H2O degradation of fibrin clots Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 Fibrin + H2O fibrinolytic activity Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 fibrinogen + H2O bovine substrate Agkistrodon contortrix contortrix fibrin + propeptide
-
 ?
3.4.24.72 fibrinogen + H2O bovine substrate, fibrinogenolytic activity Agkistrodon contortrix contortrix fibrin + propeptide
-
 ?
3.4.24.72 additional information the enzyme is a nonhemorrhagic zinc metalloprotease Agkistrodon contortrix contortrix ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.72 snake venom fibrinolytic enzyme
-
 Agkistrodon contortrix contortrix

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.72 37
-
 assay at Agkistrodon contortrix contortrix

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.72 7.5
-
 assay at Agkistrodon contortrix contortrix