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Literature summary extracted from

  • Tan, H.; Wang, J.; Zhao, Z.K.
    Purification and refolding optimization of recombinant bovine enterokinase light chain overexpressed in Escherichia coli (2007), Protein Expr. Purif., 56, 40-47.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.9 biotechnology study presents a simple and cost-effective procedure for a large-scale production Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.9 expression in Escherichia coli as glutathione S-transferase-fusion protein, the gene is a Pro82Arg/Glu176Asp variant of the known bovine EK Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.9 26000
-
 SDS-PAGE, MALDI-TOF Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.9 Bos taurus Q6B4R4 chinese northern yellow bovine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.9 of the recombinant fusion protein using nickel affinity column chromatography, refolding and auto-catalytic cleavage Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.9 duodenum
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.9 glutathione S-transferase-enterokinase + H2O GST-EK harboring an EK site Bos taurus enterokinase + glutathione S-transferase
-
 ?
3.4.21.9 GST-GFPuv + H2O harboring an EK site between GST and the green fluorescent protein GFPuv Bos taurus GST + GFPuv
-
 ?
3.4.21.9 trypsinogen + H2O
-
 Bos taurus trypsin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.9 enterokinase EK Bos taurus