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Literature summary extracted from
- Expression, purification, and activity identification of alfimeprase in Pichia pastoris (2007), Protein Expr. Purif., 54, 240-246.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.24.72 | gene alf, high level expression of His-tagged alfimeprase in Pichia pastoris strains of GS115 and KM71 by methanol induction, secretion of the recombinant enzyme to the cell culture medium | Agkistrodon contortrix contortrix |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.24.72 | extracellular | - |
Agkistrodon contortrix contortrix | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.72 | 23800 | - |
x * 23800, recombinant His-tagged alfimeprase, SDS-PAGE | Agkistrodon contortrix contortrix |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.72 | Fibrin + H2O | Agkistrodon contortrix contortrix | fibrinolytic activity | ? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.72 | Agkistrodon contortrix contortrix | P28891 | gene alf | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.72 | secreted recombinant His-tagged alfimeprase from Pichia pastoris strains of GS115 and KM71 cell culture medium by immunoaffinity chromatography to 96% purity | Agkistrodon contortrix contortrix |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.24.72 | venom | - |
Agkistrodon contortrix contortrix | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.72 | Fibrin + H2O | fibrinolytic activity | Agkistrodon contortrix contortrix | ? | - |
? | |
| 3.4.24.72 | additional information | the enzyme is a nonhemorrhagic zinc metalloprotease | Agkistrodon contortrix contortrix | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.24.72 | ? | x * 23800, recombinant His-tagged alfimeprase, SDS-PAGE | Agkistrodon contortrix contortrix |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.24.72 | ALF | - |
Agkistrodon contortrix contortrix |
| 3.4.24.72 | Alfimeprase | - |
Agkistrodon contortrix contortrix |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.72 | 37 | - |
assay at | Agkistrodon contortrix contortrix |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.72 | 7.5 | - |
assay at | Agkistrodon contortrix contortrix |
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