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Literature summary extracted from

  • Yegneswaran, S.; Kojima, Y.; Nguyen, P.M.; Gale, A.J.; Heeb, M.J.; Griffin, J.H.
    Factor Va residues 311-325 represent an activated protein C binding region (2007), J. Biol. Chem., 282, 28353-28361.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.69 Phospholipids
-
 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.69 expression of wild-type an dmutant enzymes in HEK-293 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.69 S360A site-directed mutagenesis, the active site residue mutant shows no amidolytic activity Homo sapiens
3.4.21.69 S360C site-directed mutagenesis, the active site residue mutant shows no amidolytic activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.69 peptide VP311-325 a peptide comprising residues 311-325 in factor Va, potent inhibition of factor Va-dependent prothrombin activation by factor Xa in the absence of APC, the peptide also inhibits the inactivation of factor Va by APC by APC-dependent cleavage of both Arg506 and Arg306, thus inhibits cleavage of wild-type factor Va and of the mutant R306Q and R506Q, overview Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.69 Ca2+ required Homo sapiens
3.4.21.69 Mn2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.69 active factor Va + H2O Homo sapiens proteolytic inactivation inactive factor V + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.69 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.69 active factor Va + H2O proteolytic inactivation Homo sapiens inactive factor V + ?
-
 ?
3.4.21.69 active factor Va + H2O proteolytic inactivation, cleavage of the heavy chain at Arg506, Arg306, and Arg679, factor Xa protects factor Va from inactivation by APC, interaction of factor Va and APC at residues 311-325 of the substrate Homo sapiens inactive factor V + ?
-
 ?
3.4.21.69 active factor Va mutant R306Q + H2O proteolytic inactivation, cleavage of the heavy chain at Arg506 and Arg679 Homo sapiens inactive factor V mutant R306Q + ?
-
 ?
3.4.21.69 active factor Va mutant R506Q + H2O proteolytic inactivation, cleavage of the heavy chain at Arg306 and Arg679 Homo sapiens inactive factor V mutant R506Q + ?
-
 ?
3.4.21.69 active factor Va mutant R679Q + H2O proteolytic inactivation, cleavage of the heavy chain at Arg306 and Arg506 Homo sapiens inactive factor V mutant R679Q + ?
-
 ?
3.4.21.69 Z-Gly-Gly-Arg-aminomethylcoumarin + H2O a fluorogenic substrate, S-2366 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.69 Activated protein C
-
 Homo sapiens
3.4.21.69 APC
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.69 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.69 7.4
-
 assay at Homo sapiens