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Literature summary extracted from

  • Yang, L.; Bae, J.S.; Manithody, C.; Rezaie, A.R.
    Identification of a specific exosite on activated protein C for interaction with protease-activated receptor 1 (2007), J. Biol. Chem., 282, 25493-25500.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.69 vitamin K dependent on Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.69 expression of wild-type and mutant enzymes in HEK-293 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.69 E167A site-directed mutagenesis, the surface loop residue mutation eliminates the cytoprotective signaling properties of APC without affecting its anticoagulant activity, inability of E167A to exhibit significant protective activity in response to TNF-alpha-induced inflammatory events in endothelial cells Homo sapiens
3.4.21.69 E170A site-directed mutagenesis, the surface loop residue mutation eliminates the cytoprotective signaling properties of APC without affecting its anticoagulant activity, inability of E170A to exhibit significant protective activity in response to TNF-alpha-induced inflammatory events in endothelial cells Homo sapiens
3.4.21.69 S195A site-directed mutagenesis, inactive mutant Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.69 Protein C inhibitor inhibits wild-type enzyme and mutants E167A and E170A Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.69 additional information
-
 additional information steady-state kinetics Homo sapiens
3.4.21.69 0.234
-
 SpPCa pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
3.4.21.69 0.249
-
 SpPCa pH 7.4, 37°C, recombinant mutant E167A Homo sapiens
3.4.21.69 0.258
-
 SpPCa pH 7.4, 37°C, recombinant mutant E170A Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.69 extracellular
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.69 Ca2+ required Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.69 56000
-
 x * 56000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.69 active factor Va + H2O Homo sapiens proteolytic inactivation inactive factor V + domain B
-
 ?
3.4.21.69 active factor VIIIa + H2O Homo sapiens proteolytic inactivation inactive factor VIII + domain B
-
 ?
3.4.21.69 protease activated receptor 1 + H2O Homo sapiens APC exhibits cytoprotective and antiinflammatory activity through the endothelial protein C receptor-dependent cleavage of protease activated receptor 1, PAR-1, on endothelial cells ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.69 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.69 plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.69 active factor Va + H2O proteolytic inactivation Homo sapiens inactive factor V + domain B
-
 ?
3.4.21.69 active factor Va + H2O proteolytic inactivation, the basic residues of two surface loops including those on 39 and the Ca2+-binding 70-80 loops constitute interactive sites for both factors Va and VIIIa, thereby mediating the interaction of APC specifically with these procoagulant cofactors Homo sapiens inactive factor V + domain B
-
 ?
3.4.21.69 active factor VIIIa + H2O proteolytic inactivation Homo sapiens inactive factor VIII + domain B
-
 ?
3.4.21.69 active factor VIIIa + H2O proteolytic inactivation, the basic residues of two surface loops including those on 39 and the Ca2+-binding 70-80 loops constitute interactive sites for both factors Va and VIIIa, thereby mediating the interaction of APC specifically with these procoagulant cofactors Homo sapiens inactive factor VIII + domain B
-
 ?
3.4.21.69 additional information the enzyme is a vitamin K-dependent serine protease Homo sapiens ?
-
 ?
3.4.21.69 protease activated receptor 1 + H2O APC exhibits cytoprotective and antiinflammatory activity through the endothelial protein C receptor-dependent cleavage of protease activated receptor 1, PAR-1, on endothelial cells Homo sapiens ?
-
 ?
3.4.21.69 protease activated receptor 1 + H2O activation, the basic residues of surface loops are indispensable for interaction with APC, also two acidic residues on helix-162, Glu167 and Glu170, on the protease domain of APC which are required for the protease interaction with PAR-1 Homo sapiens ?
-
 ?
3.4.21.69 S2238 + H2O a commercial chromogenic substrate Homo sapiens ?
-
 ?
3.4.21.69 SpPCa + H2O a commercial tripeptidyl chromogenic substrate Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.69 ? x * 56000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.21.69 Activated protein C
-
 Homo sapiens
3.4.21.69 APC
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.69 25
-
 anticoagulation assay at Homo sapiens
3.4.21.69 37
-
 amidohydrolytic assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.69 33.1
-
 SpPCa pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
3.4.21.69 34
-
 SpPCa pH 7.4, 37°C, recombinant mutant E167A Homo sapiens
3.4.21.69 35
-
 SpPCa pH 7.4, 37°C, recombinant mutant E170A Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.69 7.4
-
 assay at Homo sapiens