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Literature summary extracted from

  • Deghorain, M.; Goffin, P.; Fontaine, L.; Mainardi, J.L.; Daniel, R.; Errington, J.; Hallet, B.; Hols, P.
    Selectivity for D-lactate incorporation into the peptidoglycan precursors of Lactobacillus plantarum: role of Aad, a VanX-like D-alanyl-D-alanine dipeptidase (2007), J. Bacteriol., 189, 4332-4337.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.13.22 overexpression is achieved by cloning aad under the control of the nisin-inducible promoter PnisA from Lactococcus lactis. The resulting plasmid (pGIM023) is introduced into Lactobacillus plantarum strain NZ7100, a WCFS1 derivative carrying the nisR and nisK regulatory genes required for nisin induction Lactiplantibacillus plantarum

Protein Variants

EC Number Protein Variants Comment Organism
3.4.13.22 additional information an aad knockout mutant (MD119) is constructed in NZ7100 using a two-step homologous recombination procedure. Aad inactivation results in the stable replacement of the aad open reading frame (encoding 180 amino acid residues out of 185) by an erythromycin resistance cassette Lactiplantibacillus plantarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.13.22 Zn2+
-
 Lactiplantibacillus plantarum

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.22 Lactiplantibacillus plantarum
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.13.22 additional information
-
 no detectable activity in aad knockout mutant (MD119) Lactiplantibacillus plantarum
3.4.13.22 0.0015
-
 in strain overexpressing aad (NZ7100(pGIM023)), in the absence of 15 mM ZnCl2 Lactiplantibacillus plantarum
3.4.13.22 0.0019
-
 wild type strain NZ7100 , in the presence of 15 mM ZnCl2 Lactiplantibacillus plantarum
3.4.13.22 0.3872
-
 in strain overexpressing aad (NZ7100(pGIM023)), in the presence of 15 mM ZnCl2 Lactiplantibacillus plantarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.22 D-Ala-D-Ala + H2O
-
 Lactiplantibacillus plantarum D-Ala + D-Ala
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.13.22 AAD
-
 Lactiplantibacillus plantarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.13.22 7
-
 assay at Lactiplantibacillus plantarum