Literature summary extracted from

Leimanis, S.; Hoyez, N.; Hubert, S.; Laschet, M.; Sauvage, E.; Brasseur, R.; Coyette, J.
PBP5 complementation of a PBP3 deficiency in Enterococcus hirae (2006), J. Bacteriol., 188, 6298-6307.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.4.17.8	gene pbp5, complementation of a PBP5-deficient Enterococcus hirae strain SL1 and a PBP3-deficient Enterococcus hirae strain SL2	Enterococcus faecium
3.4.17.8	gene pbp5, expression of wild-type and mutant enzymes in Escherichia coli, complementation of the pbp5-deficient mutant strain SL1 and of the pbp3-deficient strain SL2 by wild-type and mutant enzymes, recombinant strains, overview	Enterococcus hirae

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.4.17.8	K172N/R173N	site-directed mutagenesis, M1 mutant, the mutant enzyme shows altered complementation of the pbp3-deficient mutant strain SL2 compared to the wild-type enzyme, the mutant enzyme shows complementation of the pbp5-deficient mutant strain SL1, overview	Enterococcus hirae
3.4.17.8	K199Q	site-directed mutagenesis, M5 mutant, the mutant enzyme shows complementation of the pbp5-deficient mutant strain SL1	Enterococcus hirae
3.4.17.8	K199Q/K203Q	site-directed mutagenesis, M2 mutant, the mutant enzyme shows altered complementation of the pbp3-deficient mutant strain SL2 compared to the wild-type enzyme, the mutant enzyme shows complementation of the pbp5-deficient mutant strain SL1, overview	Enterococcus hirae
3.4.17.8	K203Q	site-directed mutagenesis, M3 mutant, the mutant enzyme shows altered complementation of the pbp3-deficient mutant strain SL2 compared to the wild-type enzyme, the mutant enzyme shows complementation of the pbp5-deficient mutant strain SL1, overview	Enterococcus hirae
3.4.17.8	R173N	site-directed mutagenesis, M4 mutant, the mutant enzyme shows complementation of the pbp5-deficient mutant strain SL1	Enterococcus hirae
3.4.17.8	S422A	site-directed mutagenesis, M6 mutant, inactive mutant	Enterococcus hirae

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.17.8	Enterococcus faecium	-	gene pbp5	-
3.4.17.8	Enterococcus hirae	-	wild-type strain ATCC 9790, gene pbp5	-

Synonyms

EC Number	Synonyms	Comment	Organism
3.4.17.8	PBP5	-	Enterococcus faecium
3.4.17.8	PBP5	-	Enterococcus hirae
3.4.17.8	penicillin-binding protein 5	-	Enterococcus faecium
3.4.17.8	penicillin-binding protein 5	-	Enterococcus hirae

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Release 2023.1 (January 2023)