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Literature summary extracted from
- Human recombinant prolidase from eukaryotic and prokaryotic sources. Expression, purification, characterization and long-term stability studies (2006), FEBS J., 273, 5466-5478.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.13.9 | expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21 (DE3) under the control of the cytomegalovirus promoter and the SP163 enhancer | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.13.9 | additional information | enzyme decficiency leads to a rare autosomal recessive disease, characterized by a wide range of clinical outcomes, including severe skin lesions, mental retardation, and infections of the respiratory tract, genotype/phenotype relationship, overview | Homo sapiens |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.13.9 | Mn2+ and GSH stabilize the untagged recombinant enzyme | Homo sapiens |
| 3.4.13.9 | PEG and glycerol stabilize the enzyme | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.13.9 | N-Benzyloxycarbonyl-L-proline | - |
Homo sapiens |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.13.9 | cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.13.9 | Ca2+ | can substitute for Mn2+ by less than 30% | Homo sapiens | |
| 3.4.13.9 | Co2+ | can substitute for Mn2+ by less than 30% | Homo sapiens | |
| 3.4.13.9 | Mg2+ | can substitute for Mn2+ by less than 30% | Homo sapiens | |
| 3.4.13.9 | Mn2+ | dependent on, best divalent cation | Homo sapiens | |
| 3.4.13.9 | Zn2+ | can substitute for Mn2+ by less than 30% | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.13.9 | 58000 | - |
x * 58000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.13.9 | additional information | Homo sapiens | prolidase deficiency causes a rare autosomal recessive disease, characterized by a wide range of clinical outcomes, including severe skin lesions, mental retardation, and infections of the respiratory tract | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.13.9 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.13.9 | recombinant N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity, on-column digestion of the N-terminal His-tag by factor Xa | Homo sapiens |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.4.13.9 | hydrolysis of Xaa-/-Pro dipeptides; also acts on aminoacyl-hydroxyproline analogs. No action on Pro-Pro | catalytic mechanism | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.13.9 | fibroblast | - |
Homo sapiens | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.13.9 | 37°C, purified recombinant enzyme, loss of almost all activity after 2 days, 70% remaining activity after one day for the detagged enzyme, 47% remaining activity for the tagged enzyme | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.13.9 | Ala-Pro + H2O | - |
Homo sapiens | Ala + Pro | - |
? | |
| 3.4.13.9 | Gly-Pro + H2O | - |
Homo sapiens | Gly + Pro | - |
? | |
| 3.4.13.9 | Leu-Pro + H2O | - |
Homo sapiens | Leu + Pro | - |
? | |
| 3.4.13.9 | additional information | prolidase deficiency causes a rare autosomal recessive disease, characterized by a wide range of clinical outcomes, including severe skin lesions, mental retardation, and infections of the respiratory tract | Homo sapiens | ? | - |
? | |
| 3.4.13.9 | additional information | prolidase is a Mn2+-dependent exo- or dipeptidase that cleaves imidodipeptides containing C-terminal proline or hydroxyproline, substrate specificity of native and recombinant enzymes, molecular modeling, overview | Homo sapiens | ? | - |
? | |
| 3.4.13.9 | Phe-Pro + H2O | - |
Homo sapiens | Phe + Pro | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.13.9 | ? | x * 58000, recombinant enzyme, SDS-PAGE | Homo sapiens |
| 3.4.13.9 | More | molecular modeling | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.13.9 | More | the enzyme is a member of the metallopeptidase family | Homo sapiens |
| 3.4.13.9 | prolidase | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.13.9 | 50 | - |
- |
Homo sapiens |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.13.9 | 37 | 50 | 50% of maximal activity at 37°C | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.13.9 | 7.8 | - |
- |
Homo sapiens |
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