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Literature summary extracted from

  • Roderfeld, M.; Weiskirchen, R.; Wagner, S.; Berres, M.L.; Henkel, C.; Groetzinger, J.; Gressner, A.M.; Matern, S.; Roeb, E.
    Inhibition of hepatic fibrogenesis by matrix metalloproteinase-9 mutants in mice (2006), FASEB J., 20, 444-454.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.35 expression of mutant enzymes in Hep-G2 cells using recombinant adenoviral vectors adenovirus-2 or adenovirus-5, secretion of the recombinant enzymes, overview Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.35 E402H/H411E site-directed mutagenesis, proteolytic inactive MMP-9 mutant, application of the MMP-9 mutant decreases portal and periportal accumulation of collagen and inhibits fibrogenesis induced by CCl4 in mice Mus musculus
3.4.24.35 E402Q site-directed mutagenesis, proteolytic inactive MMP-9 mutant, application of the MMP-9 mutant decreases portal and periportal accumulation of collagen and inhibits fibrogenesis induced by CCl4 in mice, the MMP-9 mutant suppresses transdifferentiation of hepatic stellate cells to the myofibroblast-like phenotype in vitro and in vivo. Adenoviral application of the mutant leads to increased apoptosis of activated hepatic stellate cells Mus musculus
3.4.24.35 H401A site-directed mutagenesis, proteolytic inactive MMP-9 mutant, application of the MMP-9 mutant decreases portal and periportal accumulation of collagen and inhibits fibrogenesis induced by CCl4 in mice, the MMP-9 mutant suppresses transdifferentiation of hepatic stellate cells to the myofibroblast-like phenotype in vitro and in vivo. Adenoviral application of the mutant leads to increased apoptosis of activated hepatic stellate cells Mus musculus
3.4.24.35 additional information MMP-9 mutants reduce type I collagen protein, TIMP-1, and MMP-2 expression in vivo, overview Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.35 tissue inhibitor of metalloproteinases-1 i.e. TIMP-1, plays a crucial role in the pathogenesis of hepatic fibrosis and thus may represent an important therapeutic target in the design of anti-fibrotic strategiesfor chronic liver disease, overview, molecular modeling of the three-dimensional structure of the MMP-9/TIMP-1 complex, overview Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.35 Mus musculus
-
 Balb/c mice
-
3.4.24.35 Mus musculus BALB/c
-
 Balb/c mice
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.35 recombinant secreted mutant enzymes from Hep-G2 cells by gelatin affinity chromatography to homogeneity Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.35 liver
-
 Mus musculus
-

Subunits

EC Number Subunits Comment Organism
3.4.24.35 More molecular modeling of the three-dimensional structure of the MMP-9/TIMP-1 complex, overview Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.4.24.35 matrix metalloproteinase-9
-
 Mus musculus
3.4.24.35 MMP-9
-
 Mus musculus