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Literature summary extracted from

  • Chunhachart, O.; Itoh, T.; Sukchotiratana, M.; Tanimoto, H.; Tahara, Y.
    Characterization of gamma-glutamyl hydrolase produced by Bacillus sp. isolated from Thai Thua-nao (2006), Biosci. Biotechnol. Biochem., 70, 2779-2782.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.19.9 4-hydroxymercuribenzoate 40% inhibition at 5 mM Bacillus sp. (in: Bacteria)
3.4.19.9 additional information no inhibition by EDTA, PMSF, or iodoacetic acid at 5 mM Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.19.9 additional information Ca2+, Co2+, Mg2+, Mn2+, and Ni2+ at 5 mM have little effect on enzyme activity Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.19.9 28000
-
 x * 28000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.9 Bacillus sp. (in: Bacteria)
-
 NBRC16449, CMU29, isolated from Thai Thua-nao, a natto-like fermented soybean food
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.19.9 native enzyme from culture broth Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.9 (poly-gamma-L-glutamate)n + H2O
-
 Bacillus sp. (in: Bacteria) oligo-gamma-L-glutamate
-
 ?
3.4.19.9 di-gamma-L-glutamate + H2O
-
 Bacillus sp. (in: Bacteria) L-glutamate
-
 ?
3.4.19.9 additional information the enzyme hydrolyzes synthetically hydrolyzed oligo-gamma-L-glutamates, but not oligo-gamma-D-glutamates, and degrades polyglutamic acid to a hydrolyzed product of about 20 kDa with a ratio of D- and L-glutamic acids of 70:30, thus the enzyme cleaves the bond between two L-glutamic acid residues, overview Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.19.9 oligo-gamma-L-glutamate + H2O
-
 Bacillus sp. (in: Bacteria) L-glutamate
-
 ?
3.4.19.9 tetra-gamma-L-glutamate + H2O
-
 Bacillus sp. (in: Bacteria) L-glutamate
-
 ?
3.4.19.9 tri-gamma-L-glutamate + H2O
-
 Bacillus sp. (in: Bacteria) L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.19.9 ? x * 28000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.19.9 37
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.19.9 40
-
 1 h, pH 8.0, stable Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.19.9 8
-
-
 Bacillus sp. (in: Bacteria)