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Literature summary extracted from

  • Coetzer, T.H.; Goldring, J.P.; Huson, L.E.
    Oligopeptidase B: a processing peptidase involved in pathogenesis (2008), Biochimie, 90, 336-344.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.83 cysteine
-
 Trypanosoma brucei
3.4.21.83 dithiothreitol
-
 Trypanosoma brucei
3.4.21.83 glutathione
-
 Trypanosoma brucei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.83 crystal structure solved at 2.7 A resolution Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.83 C559S mutant shows significant decrease in activation by reducing agents. This residue may thus mediate the activity enhancing effect of reducing agents Trypanosoma brucei
3.4.21.83 C597S mutant shows significant decrease in activation by reducing agents. This residue may thus mediate the activity enhancing effect of reducing agents Trypanosoma brucei
3.4.21.83 additional information cys256 is identified as the reactive cysteine residue responsible for inactivation by N-ethylmaleimide and iodoacetic acid Trypanosoma brucei
3.4.21.83 additional information mutation of Tyr452 confirmed that this residue is involved in the catalytic mechanism by stabilising the intermediate consisting of an oxoanion. Furthermore, it is shown that this oxanion binding site prevents non-productive substrate binding Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.83 3,4-dichloroisocoumarin non-peptide irreversible serine-peptidase inhibitor Trypanosoma brucei
3.4.21.83 4-(2-aminoethyl)benzenesulfonylfluoride non-peptide irreversible serine-peptidase inhibitor Trypanosoma brucei
3.4.21.83 4-methylumbelliferyl-p-guanidobenzoate active site titrant Trypanosoma brucei
3.4.21.83 antipain reversible competitive inhibitor Trypanosoma brucei
3.4.21.83 diisopropylfluorophosphate non-peptide irreversible serine-peptidase inhibitor Trypanosoma brucei
3.4.21.83 iodoacetic acid
-
 Trypanosoma brucei
3.4.21.83 leupeptin reversible competitive inhibitor Trypanosoma brucei
3.4.21.83 additional information no inhibition by N-ethylmaleimide and iodoacetic acid. This is consistent with the absence of a cysteine residue at position 256 which is replaced by His Escherichia coli
3.4.21.83 N-ethylmaleimide
-
 Trypanosoma brucei
3.4.21.83 p-chloromercuribenzoate non-peptide irreversible serine-peptidase inhibitor Trypanosoma brucei
3.4.21.83 Pentamidine reversible competitive inhibitor Trypanosoma brucei
3.4.21.83 peptidyl chloromethyl ketone irreversible peptidyl inhibitors Trypanosoma brucei
3.4.21.83 peptidyl diazomethyl ketone irreversible peptidyl inhibitors Trypanosoma brucei
3.4.21.83 peptidyl phosphonate alpha-aminoalkyl diphenyl ester irreversible peptidyl inhibitors Trypanosoma brucei
3.4.21.83 protamine reversible competitive inhibitor Trypanosoma brucei
3.4.21.83 protamines protamines, basic 30-32 residue peptides that are rich in Arg residues, are potent inhibitors of OpdB Escherichia coli
3.4.21.83 suramin partial non-competitive inhibitor Trypanosoma brucei
3.4.21.83 Tos-LysCH2Cl non-peptide irreversible serine-peptidase inhibitor Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.83 cytosol
-
 Escherichia coli 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.83 120000
-
-
 Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.83 Escherichia coli P24555
-
-
3.4.21.83 Moraxella lacunata Q59536
-
-
3.4.21.83 Rhodococcus erythropolis
-
-
-
3.4.21.83 Salmonella enterica
-
-
-
3.4.21.83 Treponema denticola
-
-
-
3.4.21.83 Trypanosoma brucei Q382P7
-
-
3.4.21.83 Trypanosoma cruzi Q4CW30
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.83 additional information
-
 the specific activity of OpdB in Escherichia coli increases under conditions where the carbon levels are limiting Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.83 additional information cleaves oxidised insulin B chain Escherichia coli ?
-
 ?
3.4.21.83 additional information OpdB does not hydrolyse protein substrates with the exception of nominal digestion of the endogenous Escherichia coli enzymes aspartokinase L-homoserine dehydrogenase and aspartokinase III Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.83 oligopeptidase B
-
 Rhodococcus erythropolis
3.4.21.83 oligopeptidase B
-
 Salmonella enterica
3.4.21.83 oligopeptidase B
-
 Treponema denticola
3.4.21.83 oligopeptidase B
-
 Moraxella lacunata
3.4.21.83 oligopeptidase B
-
 Trypanosoma brucei
3.4.21.83 oligopeptidase B
-
 Trypanosoma cruzi
3.4.21.83 oligopeptidase B
-
 Escherichia coli
3.4.21.83 OP-Tb
-
 Trypanosoma brucei
3.4.21.83 Opd B
-
 Rhodococcus erythropolis
3.4.21.83 Opd B
-
 Salmonella enterica
3.4.21.83 Opd B
-
 Treponema denticola
3.4.21.83 Opd B
-
 Moraxella lacunata
3.4.21.83 Opd B
-
 Trypanosoma cruzi
3.4.21.83 Opd B
-
 Escherichia coli
3.4.21.83 Protease II
-
 Rhodococcus erythropolis
3.4.21.83 Protease II
-
 Salmonella enterica
3.4.21.83 Protease II
-
 Treponema denticola
3.4.21.83 Protease II
-
 Moraxella lacunata
3.4.21.83 Protease II
-
 Escherichia coli
3.4.21.83 Tc 120
-
 Trypanosoma cruzi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.83 0.00000181
-
 antipain
-
 Trypanosoma brucei
3.4.21.83 0.00001
-
 protamine
-
 Trypanosoma brucei
3.4.21.83 0.00003
-
 leupeptin
-
 Trypanosoma brucei
3.4.21.83 0.0034
-
 Pentamidine
-
 Trypanosoma brucei
3.4.21.83 0.0067
-
 suramin
-
 Trypanosoma brucei