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Literature summary extracted from

  • Gasparian, M.E.; Ostapchenko, V.G.; Dolgikh, D.A.; Kirpichnikov, M.P.
    Biochemical characterization of human enteropeptidase light chain (2006), Biochemistry (Moscow), 71, 113-119.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.9 expression as fusion protein with thioredoxin in Escherichia coli Homo sapiens
3.4.21.9 expression as fusion protein with thioredoxin in Escherichia coli Bos taurus

General Stability

EC Number General Stability Organism
3.4.21.9 highly resistant to detergent to triton X-100, tween 20 and SDS Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.9 antipain 85-95% loss of activtity Homo sapiens
3.4.21.9 Aprotinin 40-90% loss of activtity Homo sapiens
3.4.21.9 benzamidine 75-92% loss of activtity Homo sapiens
3.4.21.9 bestatin 7% loss of activtity Homo sapiens
3.4.21.9 chymostatin 20% loss of activtity Homo sapiens
3.4.21.9 dithiothreitol 10% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate Homo sapiens
3.4.21.9 E-64 50-85% loss of activtity Homo sapiens
3.4.21.9 ethanol 80-40% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate Homo sapiens
3.4.21.9 imidazole 90-30% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate Homo sapiens
3.4.21.9 leupeptin 94% loss of activtity Homo sapiens
3.4.21.9 additional information EDTA not inhibitory Homo sapiens
3.4.21.9 N-tosyl-L-lysine chloromethyl ketone 13% loss of activtity Homo sapiens
3.4.21.9 NaCl at 100 and 250 mM, Gly-Asp-Asp-Asp-Asp-Lys-?-naphthylamide as substrate Homo sapiens
3.4.21.9 pepstatin 5% loss of activtity Homo sapiens
3.4.21.9 pepstatin A 5% loss of activtity Homo sapiens
3.4.21.9 PMSF 50% loss of activtity Homo sapiens
3.4.21.9 SDS 40-70% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate Homo sapiens
3.4.21.9 Soybean trypsin inhibitor 40-90% loss of activtity Homo sapiens
3.4.21.9 Triton X-100 30-80% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate Homo sapiens
3.4.21.9 Tween-20 20-75% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate Homo sapiens
3.4.21.9 Urea 50-70% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.9 0.034
-
 Gly-Asp-Asp-Asp-Asp-Lys-naphthylamide in presence of 0.02 mM CaCl2 Homo sapiens
3.4.21.9 0.067
-
 Z-Ala-Phe-Arg-nitroanilide
-
 Homo sapiens
3.4.21.9 0.076
-
 N-formyl-Ala-Phe-Arg-4-nitroanilide
-
 Homo sapiens
3.4.21.9 0.08
-
 Gly-Asp-Asp-Asp-Asp-Lys-naphthylamide in presence of 1mM EDTA Homo sapiens
3.4.21.9 0.091
-
 Z-Gly-Pro-Arg-nitroanilide
-
 Homo sapiens
3.4.21.9 0.109
-
 Z-Ala-Tyr-Arg-nitroanilide
-
 Homo sapiens
3.4.21.9 0.14
-
 thiobenzyl benzyloxycarbonyl-L-lysinate
-
 Homo sapiens
3.4.21.9 0.141
-
 Gly-Asp-Asp-Asp-Asp-Lys-naphthylamide in presence of 0.02 mM CaCl2 Bos taurus
3.4.21.9 0.167
-
 N-formyl-Ala-Phe-Lys-4-nitroanilide
-
 Homo sapiens
3.4.21.9 0.234
-
 Z-Ala-Leu-Arg-nitroanilide
-
 Homo sapiens
3.4.21.9 0.24
-
 Z-Ala-Trp-Arg-nitroanilide
-
 Homo sapiens
3.4.21.9 0.265
-
 Z-Pro-Phe-Arg-nitroanilide
-
 Homo sapiens
3.4.21.9 0.332
-
 Gly-Asp-Asp-Asp-Asp-Lys-naphthylamide in presence of 1mM EDTA Bos taurus
3.4.21.9 0.356
-
 Z-Ala-Ala-Arg-nitroanilide
-
 Homo sapiens
3.4.21.9 0.42
-
 Z-Ala-Met-Arg-nitroanilide
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.9 Ca2+ optimal at 0.02mM, inhibitory at 10 mM Homo sapiens
3.4.21.9 Ca2+ optimal at 0.02mM, inhibitory at 10 mM Bos taurus
3.4.21.9 K+ activating, thiobenzyl benzyloxycarbonyl-L-lysinate as non-specific substrate Homo sapiens
3.4.21.9 Na+ activating, thiobenzyl benzyloxycarbonyl-L-lysinate as non-specific substrate Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.9 Bos taurus
-
-
-
3.4.21.9 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.9 solubilization of inclusion bodies with 6 M guanidine-HCl, affinity chromatography on STI-agarose Homo sapiens
3.4.21.9 solubilization of inclusion bodies with 6 M guanidine-HCl, affinity chromatography on STI-agarose Bos taurus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.9 220
-
 thioredoxin-human epidermal growth factor fusion protein as substrate Bos taurus
3.4.21.9 2100
-
 thioredoxin/human epidermal growth factor fusion protein as substrate Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.4.21.9 100% activity during storage at –20°C for at least for 1.5 years Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.9 benzyloxycarbonyl-L-alanine-X-L-arginine-p-nitroanilide + H2O Z-Ala-X-Arg-pNA Homo sapiens p-nitroaniline + benzyloxycarbonyl-L-alanine-X-L-arginine
-
 ?
3.4.21.9 formyl-Ala-Phe-Arg-4-nitroanilide + H2O
-
 Homo sapiens 4-nitroaniline + formyl-Ala-Phe-Arg
-
 ?
3.4.21.9 formyl-Ala-Phe-Lys-4-nitroanilide + H2O
-
 Homo sapiens 4-nitroaniline + formyl-Ala-Phe-Lys
-
 ?
3.4.21.9 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O GD4K-na Homo sapiens Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine
-
 ?
3.4.21.9 Gly-Asp-Asp-Asp-Asp-Lys-naphthylamide + H2O GD4K-na Bos taurus naphthylamine + Gly-Asp-Asp-Asp-Asp-Lys
-
 ?
3.4.21.9 thiobenzyl benzyloxycarbonyl-L-lysinate + H2O Z-Lys-SBzl Homo sapiens 3-carboxy-4-nitrothiophenoxide + ?
-
 ?
3.4.21.9 thioredoxin-human epidermal growth factor fusion protein + H2O Trx/hEGF Homo sapiens thioredoxin + human epidermal growth factor
-
 ?
3.4.21.9 thioredoxin-human epidermal growth factor fusion protein + H2O Trx/hEGF Bos taurus thioredoxin + human epidermal growth factor
-
 ?
3.4.21.9 Z-Ala-Ala-Arg-p-nitroanilide + H2O
-
 Homo sapiens p-nitroaniline + Z-Ala-Ala-Arg
-
 ?
3.4.21.9 Z-Ala-Leu-Arg-p-nitroanilide + H2O
-
 Homo sapiens p-nitroaniline + Z-Ala-Leu-Arg
-
 ?
3.4.21.9 Z-Ala-Met-Arg-p-nitroanilide + H2O
-
 Homo sapiens p-nitroaniline + Z-Ala-Met-Arg
-
 ?
3.4.21.9 Z-Ala-Phe-Arg-p-nitroanilide + H2O
-
 Homo sapiens p-nitroaniline + Z-Ala-Phe-Arg
-
 ?
3.4.21.9 Z-Ala-Trp-Arg-p-nitroanilide + H2O
-
 Homo sapiens Z-Ala-Trp-Arg + 4-nitroaniline
-
 ?
3.4.21.9 Z-Ala-Tyr-Arg-p-nitroanilide + H2O
-
 Homo sapiens p-nitroaniline + Z-Ala-Tyr-Arg
-
 ?
3.4.21.9 Z-Gly-Pro-Arg-p-nitroanilide + H2O
-
 Homo sapiens p-nitroaniline + Z-Gly-Pro-Arg
-
 ?
3.4.21.9 Z-Pro-Phe-Arg-p-nitroanilide + H2O
-
 Homo sapiens p-nitroaniline + Z-Pro-Phe-Arg
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.9 enteropeptidase
-
 Homo sapiens
3.4.21.9 enteropeptidase
-
 Bos taurus
3.4.21.9 enteropeptidase light chain L-BEP Bos taurus
3.4.21.9 enteropeptidase light chain L-HEP Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.9 7.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.9 6 9
-
 Homo sapiens