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Literature summary extracted from

  • Zhang, W.; Shi, Q.; Meroueh, S.O.; Vakulenko, S.B.; Mobashery, S.
    Catalytic mechanism of penicillin-binding protein 5 of Escherichia coli (2007), Biochemistry, 46, 10113-10121.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.17.8 gene pbp5, expression of wild-type and mutant enzymes in strain BL21(DE3) Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.4.17.8 C115S site-directed mutagenesis, truncated mutant of PBP5, pH-dependency, kinetics, and activity compared to the wild-type enzyme Escherichia coli
3.4.17.8 K213C/C115S site-directed mutagenesis, truncated double mutant of PBP5, pH-dependency, kinetics, and activity compared to the wild-type enzyme Escherichia coli
3.4.17.8 K47C/C115S site-directed mutagenesis, truncated double mutant of PBP5, pH-dependency, kinetics, and activity compared to the wild-type enzyme Escherichia coli
3.4.17.8 additional information exchange of active site residues Lys47 and Lys213 for gamma-thialysine, pH-dependency, kinetics, and activity compared to the wild-type enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.17.8 additional information
-
 additional information kinetics of wild-type and mutant enzymes Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.17.8 additional information Escherichia coli PBP5 performs a DD-carboxypeptidase reaction on the bacterial peptidoglycan, the major constituent of the cell wall ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.8 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.8 recombinant wild-type and mutant enzymes from strain BL21(DE3) by ion exchange chromatography, gel filtration, and ultrafiltration Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.17.8 cleavage of the bond UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-6-carboxy-L-lysyl-D-alanyl-/-D-alanine catalytic mechanism involving active site residues Lys47 and Lys213, Lys47 exists in the free-base form for catalysis, Lys213 serves as an electrostatic anchor for the substrate Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.8 diacetyl-L-Lys-D-Ala-D-Ala + H2O
-
 Escherichia coli diacetyl-L-Lys-D-Ala + D-Ala
-
 ?
3.4.17.8 additional information PBP5 performs a DD-carboxypeptidase reaction on the bacterial peptidoglycan, the major constituent of the cell wall Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.17.8 PBP5
-
 Escherichia coli
3.4.17.8 penicillin-binding protein 5
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.17.8 30
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.8 9.5 10.5
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.17.8 additional information
-
 pH profile, wild-type and mutant enzymes Escherichia coli