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Literature summary extracted from

  • Keener, W.K.; Rivera, V.R.; Young, C.C.; Poli, M.A.
    An activity-dependent assay for ricin and related RNA N-glycosidases based on electrochemiluminescence (2006), Anal. Biochem., 357, 200-207.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.2.22 analysis electrochemiluminescence-based detection of RNA N-glycosidase activities with a limit of detection of 0.1 ng/ml of ricin Ricinus communis
3.2.2.22 analysis electrochemiluminescence-based detection of RNA N-glycosidase activities with a limit of detection of 0.1 ng/ml of ricin Saponaria officinalis
3.2.2.22 analysis electrochemiluminescence-based detection of RNA N-glycosidase activities with a limit of detection of 0.1 ng/ml of ricin Abrus precatorius

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.22 dithiothreitol 20 mM, 74% residual activity Ricinus communis
3.2.2.22 HCl 20 mM, 38% residual activity Ricinus communis
3.2.2.22 KCl 20 mM, 79% residual activity Ricinus communis
3.2.2.22 MgCl2 10 mM, 65% residual activity Ricinus communis
3.2.2.22 NaCl 20 mM, 90% residual activity Ricinus communis
3.2.2.22 NaOH 20 mM, 41% residual activity Ricinus communis

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.22 Abrus precatorius
-
-
-
3.2.2.22 Ricinus communis
-
 isoforms agglutinin, ricin
-
3.2.2.22 Saponaria officinalis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.22 GdAGA + H2O substrate forms a hairpin structure, clevage in presence of N,N'-dimethylethylendiamine Ricinus communis ?
-
 ?
3.2.2.22 GdAGA + H2O substrate forms a hairpin structure, clevage in presence of N,N'-dimethylethylendiamine Saponaria officinalis ?
-
 ?
3.2.2.22 GdAGA + H2O substrate forms a hairpin structure, clevage in presence of N,N'-dimethylethylendiamine Abrus precatorius ?
-
 ?
3.2.2.22 additional information no substrate: GAGA, GdIGA, GdUGA Ricinus communis ?
-
 ?
3.2.2.22 additional information no substrate: GAGA, GdIGA, GdUGA Saponaria officinalis ?
-
 ?
3.2.2.22 additional information no substrate: GAGA, GdIGA, GdUGA Abrus precatorius ?
-
 ?