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Literature summary extracted from

  • Flodrova, D.; Dzurova, M.; Liskova, D.; Mohand, F.A.; Mislovicova, D.; Malovikova, A.; Voburka, Z.; Omelkova, J.; Stratilova, E.
    Pectate hydrolases of parsley (Petroselinum crispum) roots (2007), Z. Naturforsch. C, 62, 382-388.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Petroselinum crispum
-
-
-
3.2.1.67 Petroselinum crispum
-
 cv. Olomoucka ´ dlouha´
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15 by gel filtration and affinity chromatographies, to homogeneity Petroselinum crispum
3.2.1.67
-
 Petroselinum crispum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.15 pulp
-
 Petroselinum crispum
-
3.2.1.15 root soluble fraction Petroselinum crispum
-
3.2.1.67 root
-
 Petroselinum crispum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 oligogalacturonic acid + H2O affinity of OGHs increase with increasing degree of polymerization of the substrate, but the increase of the maximal rate is stopped by OGH when polymeric substrate is used. Form with pH optimum 4.6 prefers higher oligogalacturonates Petroselinum crispum ?
-
 ?
3.2.1.15 pectate + H2O
-
 Petroselinum crispum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.15 OGH
-
 Petroselinum crispum
3.2.1.15 oligogalacturonate hydrolase
-
 Petroselinum crispum
3.2.1.15 pectate hydrolase
-
 Petroselinum crispum
3.2.1.67 exopolygalacturonase
-
 Petroselinum crispum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.15 additional information
-
 between 60 and 70°C Petroselinum crispum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.15 55
-
 100% recovery of activity after 2 h Petroselinum crispum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 additional information
-
 three forms with pH optima 3.6, 4.2 and 4.6 Petroselinum crispum
3.2.1.67 5.2
-
-
 Petroselinum crispum