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Literature summary extracted from

  • Yu, X.; Li, Y.; Zhou, S.; Zheng, Y.
    Synthesis of propyl gallate by mycelium-bound tannase from Aspergillus niger in organic solvent (2007), World J. Microbiol. Biotechnol., 23, 1091-1098.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.20 1-propanol activates at a concentration of 3.6-7.3% v/v, at higher concentration it inhibits the propyl gallate synthesis reaction causing disruption of essential membrane functions and denaturation of enzyme Aspergillus niger
3.1.1.20 acetone
-
 Aspergillus niger
3.1.1.20 Benzene optimum enzyme concentration should be 0.1 g mycelial dry weight per 10 ml benzene Aspergillus niger
3.1.1.20 gallic acid activates at a concentration up to 0.005 mM, at higher concentration it inhibits the propyl gallate synthesis reaction Aspergillus niger
3.1.1.20 hexane
-
 Aspergillus niger
3.1.1.20 additional information poor activating effects by octane and dodecane, tannic acid induces the enzyme, effects of organic solvents on propyl gallate production by the enzyme, and effect of water content, overview Aspergillus niger
3.1.1.20 N,N-Dimethylformamide
-
 Aspergillus niger
3.1.1.20 Pyridine
-
 Aspergillus niger

Application

EC Number Application Comment Organism
3.1.1.20 synthesis the mycelium-bound enzyme is useful as biocatalyst in a whole cell system at proper conditions to higher conversion of propyl gallate, the method could also reduce the cost and the time for the immobilization process Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.20 1-propanol activates at a concentration of 3.6-7.3% v/v, at higher concentration it inhibits the propyl gallate synthesis reaction causing disruption of essential membrane functions and denaturation of enzyme Aspergillus niger
3.1.1.20 gallic acid activates at a concentration up to 0.005 mM, at higher concentration it inhibits the propyl gallate synthesis reaction Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.20 tannic acid + H2O Aspergillus niger
-
 gallic acid + ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.20 additional information effects of organic solvents on propyl gallate production by the enzyme, overview Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Aspergillus niger
-
 inducible enzyme
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.20 mycelium mycelium-bound tannase Aspergillus niger
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 1-propanol + gallic acid the reaction takes place in organic solvents, best in benzene, effect of water content, overview Aspergillus niger propyl gallate + H2O
-
 r
3.1.1.20 tannic acid + H2O
-
 Aspergillus niger gallic acid + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 tannin acyl hydrolase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 40
-
-
 Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.20 30 50
-
 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 5
-
-
 Aspergillus niger