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Literature summary extracted from

  • Nagaraju, S.; Devaraja, S.; Kemparaju, K.
    Purification and properties of hyaluronidase from Hippasa partita (funnel web spider) venom gland extract (2007), Toxicon, 50, 383-393.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.35 NaCl optimal activity in the presence of 300 mM NaCl Hippasa partita

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.35 Ca2+ inhibition at 300 mM Hippasa partita
3.2.1.35 Mg2+ inhibition at 300 mM Hippasa partita
3.2.1.35 Zn2+ inhibition at 300 mM Hippasa partita

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.35 K+
-
 Hippasa partita
3.2.1.35 additional information not activated by Cl- Hippasa partita
3.2.1.35 Na+
-
 Hippasa partita

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.35 42260
-
 MALDI-TOF mass spectrometry Hippasa partita

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.35 Hippasa partita
-
 funnel web spider
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.35 Sephadex G-100 column chromatography and CM-Sephadex C-25 column chromatography Hippasa partita

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.35 venom
-
 Hippasa partita
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.35 0.00184
-
 venom gland extract, at 37°C Hippasa partita
3.2.1.35 0.03767
-
 after 20.4fold purification, at 37°C Hippasa partita

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.35 hyaluronan + 2 H2O absolute specificity for hyaluronan Hippasa partita N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
 ?
3.2.1.35 additional information chondroitin, chondroitin sulfate A, chondroitin sulfate B, chondroitin sulfate C, chondroitin sulfate D, heparin, and chitosan are no substrates Hippasa partita ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.35 Hyal
-
 Hippasa partita
3.2.1.35 hyaluronidase
-
 Hippasa partita

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.35 37
-
-
 Hippasa partita

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.35 5.8
-
-
 Hippasa partita