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Literature summary extracted from

  • Pilipenko, O.S.; Atyaksheva, L.F.; Poltorak, O.M.; Chukhrai, E.S.
    Dissociation and catalytic activity of oligomer forms of beta-galactosidases (2007), Russ. J. Phys. Chem. A, 81, 990-994.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 additional information
-
 additional information kinetics Escherichia coli
3.2.1.23 additional information
-
 additional information kinetics Bos taurus
3.2.1.23 additional information
-
 additional information kinetics Kluyveromyces marxianus
3.2.1.23 additional information
-
 additional information kinetics Penicillium canescens
3.2.1.23 0.093
-
 2-nitrophenyl-beta-D-galactopyranoside tetrameric enzyme, 25°C Escherichia coli
3.2.1.23 1
-
 2-nitrophenyl-beta-D-galactopyranoside dimeric enzyme, 25°C Kluyveromyces marxianus
3.2.1.23 1
-
 2-nitrophenyl-beta-D-galactopyranoside tetrameric enzyme, 25°C Penicillium canescens
3.2.1.23 2.5
-
 2-nitrophenyl-beta-D-galactopyranoside 25°C Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 12000
-
 1 * 90000 + 1 * 12000 Kluyveromyces marxianus
3.2.1.23 41000
-
 1 * 41000 Bos taurus
3.2.1.23 90000
-
 1 * 90000 + 1 * 12000 Kluyveromyces marxianus
3.2.1.23 116000
-
 4 * 116000 Escherichia coli
3.2.1.23 116000
-
 2 * 116000 Escherichia coli
3.2.1.23 203000
-
 dimeric enzyme form Kluyveromyces marxianus
3.2.1.23 220000 280000 dimeric enzyme form Penicillium canescens
3.2.1.23 232000
-
 dimeric enzyme form Escherichia coli
3.2.1.23 440000 560000 tetrameric enzyme form Penicillium canescens
3.2.1.23 464000
-
 tetrameric enzyme form Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Bos taurus
-
-
-
3.2.1.23 Escherichia coli
-
-
-
3.2.1.23 Kluyveromyces marxianus
-
-
-
3.2.1.23 Penicillium canescens
-
-
-
3.2.1.23 Penicillium canescens F-178
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.23 commercial preparation
-
 Escherichia coli
-
3.2.1.23 commercial preparation
-
 Bos taurus
-
3.2.1.23 commercial preparation
-
 Kluyveromyces marxianus
-
3.2.1.23 liver commercial preparation Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 additional information
-
-
 Escherichia coli
3.2.1.23 additional information
-
-
 Bos taurus
3.2.1.23 additional information
-
-
 Kluyveromyces marxianus
3.2.1.23 additional information
-
-
 Penicillium canescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Escherichia coli 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Bos taurus 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Kluyveromyces marxianus 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Penicillium canescens 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Penicillium canescens F-178 2-nitrophenol + beta-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 dimer 1 * 90000 + 1 * 12000 Kluyveromyces marxianus
3.2.1.23 dimer 2 * 11000-140000 Penicillium canescens
3.2.1.23 dimer 2 * 116000 Escherichia coli
3.2.1.23 monomer 1 * 41000 Bos taurus
3.2.1.23 More other oligomer forms of the enzyme are inactive Escherichia coli
3.2.1.23 More other oligomer forms of the enzyme are inactive Kluyveromyces marxianus
3.2.1.23 More other oligomer forms of the enzyme are inactive Penicillium canescens
3.2.1.23 tetramer 4 * 116000 Escherichia coli
3.2.1.23 tetramer 4 * 11000-140000 Penicillium canescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 25
-
 assay at Escherichia coli
3.2.1.23 25
-
 assay at Bos taurus
3.2.1.23 25
-
 assay at Kluyveromyces marxianus
3.2.1.23 25
-
 assay at Penicillium canescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.23 4 45
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.23 0.01 0.08 2-nitrophenyl-beta-D-galactopyranoside 25°C Bos taurus
3.2.1.23 45.4
-
 2-nitrophenyl-beta-D-galactopyranoside dimeric enzyme, 25°C Kluyveromyces marxianus
3.2.1.23 45.4
-
 2-nitrophenyl-beta-D-galactopyranoside dimeric enzyme, 25°C Penicillium canescens
3.2.1.23 180
-
 2-nitrophenyl-beta-D-galactopyranoside dimeric enzyme, 25°C Escherichia coli
3.2.1.23 400
-
 2-nitrophenyl-beta-D-galactopyranoside tetrameric enzyme, 25°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 4.2 4.5
-
 Penicillium canescens
3.2.1.23 7 7.5
-
 Escherichia coli
3.2.1.23 7 7.5
-
 Bos taurus
3.2.1.23 7.2 7.5
-
 Kluyveromyces marxianus