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Literature summary extracted from

  • Fibriansah, G.; Masuda, S.; Koizumi, N.; Nakamura, S.; Kumasaka, T.
    The 1.3 A crystal structure of a novel endo-beta-1,3-glucanase of glycoside hydrolase family 16 from alkaliphilic Nocardiopsis sp. strain F96 (2007), Proteins Struct. Funct. Bioinform., 69, 683-690.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 expressed in Escherichia coli strain B834(DE3) Nocardiopsis sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.39 hanging drop vapour diffusion method with 0.1 M Tris-HCl (pH 8.0), 1.5 M ammonium sulfate, 0.1 M sodium chloride, and 1% (v/v) ethanol Nocardiopsis sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.39 W118A the mutant almost abolishes its activity Nocardiopsis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.39 3,4-epoxybutyl-beta-D-cellobioside
-
 Nocardiopsis sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.39 Ca2+ one calcium ion is bound on the convex face of the protein, suggesting to play a role in stabilizing the protein structure Nocardiopsis sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Nocardiopsis sp.
-
-
-
3.2.1.39 Nocardiopsis sp. F96
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39
-
 Nocardiopsis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 laminarin + H2O
-
 Nocardiopsis sp. D-glucose + ?
-
 ?
3.2.1.39 laminarin + H2O
-
 Nocardiopsis sp. F96 D-glucose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 endo-beta-1,3-glucanase
-
 Nocardiopsis sp.