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Literature summary extracted from

  • Rao, K.N.; Bonanno, J.B.; Burley, S.K.; Swaminathan, S.
    Crystal structure of glycerophosphodiester phosphodiesterase from Agrobacterium tumefaciens by SAD with a large asymmetric unit (2006), Proteins Struct. Funct. Bioinform., 65, 514-518.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.46 expressed as His-tag fusion protein in Escherichia coli TOP10 cells Agrobacterium tumefaciens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.46 sitting drop vapor diffusion method Agrobacterium tumefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.46 27300
-
 6 * 27300, crystal structure analysis Agrobacterium tumefaciens
3.1.4.46 27300
-
 x * 27300, gel filtration experiments with an estimated molecular weight of approximately 160000 Da indicates a higher number of monomers than found in crystals Agrobacterium tumefaciens
3.1.4.46 160000
-
 a molecular weight of approximately 160000 Da estimated by gel filtration Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.46 glycerophosphodiester + H2O Agrobacterium tumefaciens
-
 glycerol 3-phosphate + alcohol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.46 Agrobacterium tumefaciens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.46 recombinant protein using His-tag Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.46 glycerophosphodiester + H2O
-
 Agrobacterium tumefaciens glycerol 3-phosphate + alcohol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.46 ? x * 27300, gel filtration experiments with an estimated molecular weight of approximately 160000 Da indicates a higher number of monomers than found in crystals Agrobacterium tumefaciens
3.1.4.46 hexamer 6 * 27300, crystal structure analysis Agrobacterium tumefaciens

Synonyms

EC Number Synonyms Comment Organism
3.1.4.46 GDPD
-
 Agrobacterium tumefaciens