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Literature summary extracted from
- The crystal structure of XC847 from Xanthomonas campestris: A 3-5 oligoribonuclease of DnaQ fold family with a novel opposingly shifted helix (2006), Proteins Struct. Funct. Bioinform., 65, 1036-1040.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.13.3 | into pMCSG7 vector | Xanthomonas campestris |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.1.13.3 | by sitting-drop vapor diffusion method, to a resolution of 2.1 A, orn monomer comprises 9 alpha-helices and 5 beta-strands, helix H is oriented opposingly from similar helices in all reported 3'-5' DNases discovered so far, possibly to prevent steric hindrance of accommodating oligoribonucleotide substrates. Amino acids Leu135, Leu143, Ile140, and Leu174 form a hydrophobic cluster, side chains of Glu142/Arg133 and Thr139/Asp136 form salt bridges and H-bonds in the dimer interface, while side chains of Arg145, and Lys157 interact with the ribose 2'-OH or phosphate oxygen atoms of U5 substrates | Xanthomonas campestris |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.13.3 | Mg2+ | one magnesium ion, coordinating well with the DEDDh active site carboxylate groups of Asp15, Glu17, Asp166, and the oxygen atom of U51Op group | Xanthomonas campestris |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.13.3 | Xanthomonas campestris | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.13.3 | more than 99% pure | Xanthomonas campestris |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.13.3 | homodimer | crystallography | Xanthomonas campestris |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.13.3 | XC847 | - |
Xanthomonas campestris |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.13.3 | additional information | - |
active site highly acidic and substrate binding site highly basic | Xanthomonas campestris |
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Release 2023.1 (January 2023)
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