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Literature summary extracted from

  • Onitsuka, M.; Kamikubo, H.; Yamazaki, Y.; Kataoka, M.
    Mechanism of induced folding: Both folding before binding and binding before folding can be realized in staphylococcal nuclease mutants (2008), Proteins, 72, 837-847.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.31.1 expression of wild type and mutant proteins in Escherichia coli BL21(DE3), formation of inclusion bodies Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.31.1 DELTA140-149 deletion of the 10 C-terminal residues, mutant proteins are in a non-native or disordered state under physiological conditions, folding is induced by addition of an inhibitor or substrate Staphylococcus aureus
3.1.31.1 INS33A34 insertion of an alanine between residues 33 and 34, mutant proteins are in a non-native or disordered state under physiological conditions, folding is induced by addition of an inhibitor or substrate Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.31.1 adenosine 3',5'-diphosphate able to induce folding of mutant proteins into the native state Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.1.31.1 Staphylococcus aureus
-
 native state protein and engineered proteins showing a disordered state under physiological conditions
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.31.1 recombinant proteins Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.31.1 ssDNA + H2O single stranded salmon sperm DNA Staphylococcus aureus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.31.1 SNase
-
 Staphylococcus aureus
3.1.31.1 staphylococcal nuclease
-
 Staphylococcus aureus