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Literature summary extracted from

  • Young Park, A.; Elvin, C.M.; Hamdan, S.M.; Wood, R.J.; Liyou, N.E.; Hamwood, T.E.; Jennings, P.A.; Dixon, N.E.
    Hydrolysis of the 5-p-nitrophenyl ester of TMP by oligoribonucleases (ORN) from Escherichia coli, Mycobacterium smegmatis, and human (2008), Protein Expr. Purif., 57, 180-187.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.13.3 expressed as N-terminal His6-tagged ORN enzyme under control of the bacteriophage T7 promoter in Escherichia coli BL21(kDE3)/pLysS using derivatives of the expression vector pETMCSI Escherichia coli
3.1.13.3 expressed as N-terminal His6-tagged ORN enzyme under control of the bacteriophage T7 promoter in Escherichia coli BL21(kDE3)/pLysS using derivatives of the expression vector pETMCSI Homo sapiens
3.1.13.3 expressed as N-terminal His6-tagged ORN enzyme under control of the bacteriophage T7 promoter in Escherichia coli BL21(kDE3)/pLysS using derivatives of the expression vector pETMCSI Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.13.3 Ni2+ effective competitive inhibitor Escherichia coli
3.1.13.3 Ni2+ effective competitive inhibitor Homo sapiens
3.1.13.3 Ni2+ effective competitive inhibitor Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.13.3 0.27
-
 5'-p-nitrophenyl-TMP in the presence of 3 mM Mn2+ Homo sapiens
3.1.13.3 0.28
-
 5'-p-nitrophenyl-TMP in the presence of 3 mM Mn2+ Mycolicibacterium smegmatis
3.1.13.3 0.46
-
 5'-p-nitrophenyl-TMP in the presence of 1 mM Mn2+ Mycolicibacterium smegmatis
3.1.13.3 0.54
-
 5'-p-nitrophenyl-TMP in the presence of 3 mM Mn2+ Escherichia coli
3.1.13.3 0.85
-
 5'-p-nitrophenyl-TMP in the presence of 1 mM Mn2+ Homo sapiens
3.1.13.3 1.9
-
 5'-p-nitrophenyl-TMP in the presence of 1 mM Mn2+ Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.13.3 Mg2+
-
 Escherichia coli
3.1.13.3 Mg2+
-
 Homo sapiens
3.1.13.3 Mg2+
-
 Mycolicibacterium smegmatis
3.1.13.3 Mn2+ preferred over Mg2+ Escherichia coli
3.1.13.3 Mn2+ preferred over Mg2+ Homo sapiens
3.1.13.3 Mn2+ preferred over Mg2+ Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.13.3 21638
-
 2 * 21638, electrospray ionisation mass spectrometry Escherichia coli
3.1.13.3 24576
-
 2 * 24576, electrospray ionisation mass spectrometry Homo sapiens
3.1.13.3 24637
-
 2 * 24637, electrospray ionisation mass spectrometry Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
3.1.13.3 Escherichia coli
-
-
-
3.1.13.3 Homo sapiens
-
-
-
3.1.13.3 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.13.3 on Ni-NTA column and by gel filtration, more than 95% pure Escherichia coli
3.1.13.3 on Ni-NTA column and by gel filtration, more than 95% pure Homo sapiens
3.1.13.3 on Ni-NTA column and by gel filtration, more than 95% pure Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.13.3 1.77
-
 purified enzyme Escherichia coli
3.1.13.3 7.1
-
 purified enzyme Homo sapiens
3.1.13.3 14.9
-
 purified enzyme Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.13.3 5'-p-nitrophenyl-TMP + H2O
-
 Escherichia coli p-nitrophenol + TMP
-
 ?
3.1.13.3 5'-p-nitrophenyl-TMP + H2O
-
 Homo sapiens p-nitrophenol + TMP
-
 ?
3.1.13.3 5'-p-nitrophenyl-TMP + H2O
-
 Mycolicibacterium smegmatis p-nitrophenol + TMP
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.13.3 homodimer 2 * 21638, electrospray ionisation mass spectrometry Escherichia coli
3.1.13.3 homodimer 2 * 24576, electrospray ionisation mass spectrometry Homo sapiens
3.1.13.3 homodimer 2 * 24637, electrospray ionisation mass spectrometry Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
3.1.13.3 ORN
-
 Escherichia coli
3.1.13.3 ORN
-
 Homo sapiens
3.1.13.3 ORN
-
 Mycolicibacterium smegmatis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.13.3 25
-
-
 Escherichia coli
3.1.13.3 25
-
-
 Homo sapiens
3.1.13.3 25
-
-
 Mycolicibacterium smegmatis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.13.3 0.03 0.55 5'-p-nitrophenyl-TMP in the presence of 3 mM Mn2+ Homo sapiens
3.1.13.3 0.04 1.97 5'-p-nitrophenyl-TMP in the presence of 1 mM Mn2+ Homo sapiens
3.1.13.3 0.04 1.97 5'-p-nitrophenyl-TMP in the presence of 3 mM Mn2+ Mycolicibacterium smegmatis
3.1.13.3 0.057 0.65 5'-p-nitrophenyl-TMP in the presence of 1 mM Mn2+ Mycolicibacterium smegmatis
3.1.13.3 2.15
-
 5'-p-nitrophenyl-TMP in the presence of 1 mM Mn2+ Escherichia coli
3.1.13.3 2.3
-
 5'-p-nitrophenyl-TMP in the presence of 3 mM Mn2+ Escherichia coli
3.1.13.3 7.417
-
 5'-p-nitrophenyl-TMP in the presence of 3 mM Mn2+ Homo sapiens
3.1.13.3 8.767
-
 5'-p-nitrophenyl-TMP in the presence of 1 mM Mn2+ Homo sapiens
3.1.13.3 9.317
-
 5'-p-nitrophenyl-TMP in the presence of 3 mM Mn2+ Mycolicibacterium smegmatis
3.1.13.3 10.88
-
 5'-p-nitrophenyl-TMP in the presence of 1 mM Mn2+ Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.13.3 8
-
-
 Escherichia coli
3.1.13.3 8
-
-
 Homo sapiens
3.1.13.3 8
-
-
 Mycolicibacterium smegmatis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.13.3 0.022
-
 Ni2+
-
 Homo sapiens
3.1.13.3 0.039
-
 Ni2+
-
 Escherichia coli
3.1.13.3 0.24
-
 Ni2+
-
 Mycolicibacterium smegmatis