Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yoneyama, T.; Taira, M.; Suzuki, T.; Nakamura, M.; Niwa, K.; Watanabe, T.; Ohyama, T.
    Expression and characterization of a recombinant unique acid phosphatase from kidney bean hypocotyl exhibiting chloroperoxidase activity in the yeast Pichia pastoris (2007), Protein Expr. Purif., 53, 31-39.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.2 EDTA 104% activity at 0.1 mM Phaseolus vulgaris
3.1.3.2 additional information not affected by citrate Phaseolus vulgaris
3.1.3.2 NaF 114% activity at 0.01 mM Phaseolus vulgaris
3.1.3.2 Tartrate 110% activity at 0.1 mM Phaseolus vulgaris

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2 expressed in Pichia pastoris Phaseolus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 Mn2+ 94% residual activity at 0.1 mM Phaseolus vulgaris
3.1.3.2 Mo6+ 13% residual activity at 0.1 mM Phaseolus vulgaris
3.1.3.2 VO43- 5% residual activity at 0.01 mM Phaseolus vulgaris
3.1.3.2 Zn2+ 70% residual activity at 0.1 mM Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.56
-
 p-nitrophenyl phosphate wild type enzyme Phaseolus vulgaris
3.1.3.2 0.61
-
 p-nitrophenyl phosphate recombinant enzyme Phaseolus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Fe3+ 108% activity at 0.01 mM Phaseolus vulgaris
3.1.3.2 Mn2+ 106% activity at 0.01 mM Phaseolus vulgaris
3.1.3.2 Zn2+ 101% activity at 0.01 mM Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 45000
-
 1 * 56000 + 1 * 45000, gel filtration, recombinant enzyme Phaseolus vulgaris
3.1.3.2 56000
-
 1 * 56000 + 1 * 45000, gel filtration, recombinant enzyme Phaseolus vulgaris
3.1.3.2 110000
-
 gel filtration, recombinant enzyme Phaseolus vulgaris
3.1.3.2 120000
-
 SDS-PAGE, recombinant enzyme Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Phaseolus vulgaris
-
 variety kidney bean, cultivar Ohfuku
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.2 glycoprotein
-
 Phaseolus vulgaris

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 ProBond resin nickel affinity column chromatography Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 1.3
-
 after 1fold purification, using p-nitrophenyl phosphate as a substrate Phaseolus vulgaris
3.1.3.2 32.2
-
 after 25fold purification, using p-nitrophenyl phosphate as a substrate Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 2-phosphoglycerate + H2O 32% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris glycerol + phosphate
-
 ?
3.1.3.2 3-phosphoglycerate + H2O 34% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris glycerol + phosphate
-
 ?
3.1.3.2 ADP + H2O 54% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris AMP + phosphate
-
 ?
3.1.3.2 alpha-naphthylphosphate + H2O 32% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris alpha-naphthol + phosphate
-
 ?
3.1.3.2 AMP + H2O 13% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris adenosine + phosphate
-
 ?
3.1.3.2 ATP + H2O 40% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris ADP + phosphate
-
 ?
3.1.3.2 beta-naphthylphosphate + H2O 59% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris beta-naphthol + phosphate
-
 ?
3.1.3.2 D-glucose 1-phosphate + H2O 58% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris D-glucose + phosphate
-
 ?
3.1.3.2 D-glucose 6-phosphate + H2O 46% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris D-glucose + phosphate
-
 ?
3.1.3.2 diphosphate + H2O 65% activity compared to phosphoenolpyruvate Phaseolus vulgaris 2 phosphate
-
 ?
3.1.3.2 GTP + H2O 16% activity compared to phosphoenolpyruvate Phaseolus vulgaris GDP + phosphate
-
 ?
3.1.3.2 additional information the enzyme shows chloroperoxidase activity after the addition of orthovanadate, no activity with bis-p-nitrophenyl phosphate Phaseolus vulgaris ?
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O 100% activity Phaseolus vulgaris p-nitrophenol + phosphate
-
 ?
3.1.3.2 phosphoenolpyruvate + H2O 67% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris pyruvate + phosphate
-
 ?
3.1.3.2 phosphothreonine + H2O 42% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris threonine + phosphate
-
 ?
3.1.3.2 phosphotyrosine + H2O 63% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris tyrosine + phosphate
-
 ?
3.1.3.2 phytate + H2O 16% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris ? + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 dimer 1 * 56000 + 1 * 45000, gel filtration, recombinant enzyme Phaseolus vulgaris

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 KhACP shows also chloroperoxidase activity Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 6
-
-
 Phaseolus vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.2 4 7
-
 Phaseolus vulgaris