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Literature summary extracted from
- Purification and characterization of carbonic anhydrase of rice (Oryza sativa L.) expressed in Escherichia coli (2007), Protein Expr. Purif., 52, 379-383.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.1 | expression as a glutathione-S-transferase fusion protein in Escherichia coli | Oryza sativa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.1 | acetazolamide | - |
Oryza sativa |  |
| 4.2.1.1 | Br- | - |
Oryza sativa | |
| 4.2.1.1 | Cl- | - |
Oryza sativa | |
| 4.2.1.1 | I- | - |
Oryza sativa | |
| 4.2.1.1 | N3- | - |
Oryza sativa | |
| 4.2.1.1 | NO3- | - |
Oryza sativa | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.1 | Zn2+ | addition of zinc improves the CO2 hydration activity of the rice CA produced by Escherichia coli | Oryza sativa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.1 | 29000 | - |
x * 29000, SDS-PAGE | Oryza sativa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.1 | Oryza sativa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.1 | recombinant | Oryza sativa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.1 | additional information | - |
- |
Oryza sativa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.1 | CO2 + H2O | - |
Oryza sativa | H2CO3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.1 | ? | x * 29000, SDS-PAGE | Oryza sativa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.1 | 110000 | - |
CO2 | - |
Oryza sativa | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.1 | 0.0026 | - |
acetazolamide | 25°C, pH 7.4 | Oryza sativa | |
| 4.2.1.1 | 0.0037 | - |
N3- | 25°C, pH 7.4 | Oryza sativa | |
| 4.2.1.1 | 0.04 | - |
NO3- | 25°C, pH 7.4 | Oryza sativa | |
| 4.2.1.1 | 0.14 | - |
I- | 25°C, pH 7.4 | Oryza sativa | |
| 4.2.1.1 | 2.5 | - |
Br- | 25°C, pH 7.4 | Oryza sativa | |
| 4.2.1.1 | 38 | - |
Cl- | 25°C, pH 7.4 | Oryza sativa | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 4.2.1.1 | Oryza sativa | - |
- |
8.41 |
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