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Literature summary extracted from

  • Lee, C.N.; Lin, J.W.; Chow, T.Y.; Tseng, Y.H.; Weng, S.F.
    A novel lysozyme from Xanthomonas oryzae phage varphiXo411 active against Xanthomonas and Stenotrophomonas (2006), Protein Expr. Purif., 50, 229-237.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.17 agriculture Lys411H is the first lysozyme active against both Xanthomonas and Stenotrophomonas maltophilia. This suggests that Lys411 can be a candidate to be developed into a therapeutic agent for treating Stenotrophomonas maltophilia infections, in addition to the potential use in control of the plant diseases caused by Xanthomonas Xanthomonas phage Xo411

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.17 the His-tagged protein Lys411H is expressed in Escherichia coli mostly in inclusion bodies Xanthomonas phage Xo411

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.17 CoCl2 1 mM Xanthomonas phage Xo411
3.2.1.17 EDTA at 10 and 20 mM causes 15% and 43% reduction of the enzyme activity Xanthomonas phage Xo411
3.2.1.17 FeCl3 1 mM Xanthomonas phage Xo411
3.2.1.17 NiCl 1 mM Xanthomonas phage Xo411

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.17 Cu2+ 1.0 mM, activity is increased by 54% Xanthomonas phage Xo411

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.17 Xanthomonas phage Xo411 A1BPG9 bacteriophage of Xanthomonas oryzae pv. oryzae
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.17 the insoluble protein is solubilized in urea and purified by passing through a His-bind column, and the lytic activity is then restored by a refolding process Xanthomonas phage Xo411

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.17 additional information
-
 7400 U/mg. One unit of enzyme is defined as the amounts that caused the decrease of 0.001 units of absorbance at 550 nM per min Xanthomonas phage Xo411

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.17 peptidoglycan + H2O the enzyme is active against both Xanthomonas and Stenotrophomonas maltophilia. Only a minor portion of the Escherichia coli cells is lysed. Cells of Micococcus lysodeikticus, Bacillus subtilis, Agrobacterium tumefaciens, and Psuedomonas fluorescens exhibit no signifiant lysis Xanthomonas phage Xo411 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.17 25
-
 the optimal assay conditions determined for the recombinant His-tagged protein are in 0.1M potassium phosphate buffer, pH 6.6 containing 1 mM CuCl2 at 25°C Xanthomonas phage Xo411

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.17 25 50 25°C: maximal activity, 45°C: about 90% of maximal activity, 50°C: about 40% of maximal activity Xanthomonas phage Xo411

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.17 20
-
 10 min, 3% loss of activity Xanthomonas phage Xo411
3.2.1.17 30
-
 10 min, 14% loss of activity Xanthomonas phage Xo411
3.2.1.17 40
-
 10 min, 25% loss of activity Xanthomonas phage Xo411
3.2.1.17 50
-
 10 min, 30% loss of activity Xanthomonas phage Xo411
3.2.1.17 55
-
 10 min, 66% loss of activity Xanthomonas phage Xo411

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.17 6.6
-
 the optimal assay conditions determined for the recombinant His-tagged protein are in 0.1M potassium phosphate buffer, pH 6.6 containing 1 mM CuCl2 at 25°C Xanthomonas phage Xo411

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.17 6 7 pH 6.0: about 60% of maximal activity, pH 7.0: about 55% of maximal activity Xanthomonas phage Xo411