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Literature summary extracted from
- Expression of organophosphorus hydrolase OPHC2 in Pichia pastoris: purification and characterization (2006), Protein Expr. Purif., 49, 9-14.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.8.1 | ORF ophc2, subcloning in Escherichia coli strain JM109, functional high level expression of OPHC2 in Pichia pastoris strain GS115, secretion to the culture supernatant | Pseudomonas oleovorans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.8.1 | Ca2+ | 5% inhibition at 1 mM | Pseudomonas oleovorans |  |
| 3.1.8.1 | Mn2+ | 18% inhibition at 1 mM | Pseudomonas oleovorans | |
| 3.1.8.1 | Pb2+ | 20% inhibition at 1 mM | Pseudomonas oleovorans | |
| 3.1.8.1 | Zn2+ | 11% inhibition at 1 mM | Pseudomonas oleovorans | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.8.1 | Co2+ | activates by 14.2% at 1 mM | Pseudomonas oleovorans | |
| 3.1.8.1 | additional information | no effect by Ca2+, Cr3+, Mg2+, Li+, and EDTA at 1 mM | Pseudomonas oleovorans | |
| 3.1.8.1 | Ni2+ | activates by 23.5% at 1 mM | Pseudomonas oleovorans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.8.1 | 36000 | - |
x * 36000, recombinant OPHC2, SDS-PAGE | Pseudomonas oleovorans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.8.1 | Pseudomonas oleovorans | Q5W503 | ORF ophc2 | - |
| 3.1.8.1 | Pseudomonas oleovorans C2-1 | Q5W503 | ORF ophc2 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.8.1 | recombinant OPHC2 from Pichia pastoris strain GS115 culture supernatant by gel filtration and ultrafiltration | Pseudomonas oleovorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.8.1 | methyl parathion + H2O | - |
Pseudomonas oleovorans | dimethyl thiophosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | methyl parathion + H2O | - |
Pseudomonas oleovorans C2-1 | dimethyl thiophosphate + 4-nitrophenol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.8.1 | ? | x * 36000, recombinant OPHC2, SDS-PAGE | Pseudomonas oleovorans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.8.1 | OPHC2 | - |
Pseudomonas oleovorans |
| 3.1.8.1 | organophosphorus hydrolase | - |
Pseudomonas oleovorans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.8.1 | 65 | - |
recombinant enzyme | Pseudomonas oleovorans |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.8.1 | 20 | 80 | - |
Pseudomonas oleovorans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.8.1 | 75 | - |
30 min, purified recombinant enzyme, over 60% remaining activity | Pseudomonas oleovorans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.8.1 | 9 | - |
recombinant enzyme | Pseudomonas oleovorans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.8.1 | 5 | 10 | 50% of maximal activity at pH 5.0 and below | Pseudomonas oleovorans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.8.1 | 5 | - |
30 min, purified enzyme, loss of 50% activity at pH 5.0 and below | Pseudomonas oleovorans |
| 3.1.8.1 | 6 | 11 | 30 min, purified enzyme, over 70% remaining activity | Pseudomonas oleovorans |
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