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Literature summary extracted from

  • Ammendola, S.; Raucci, G.; Incani, O.; Mele, A.; Tramontano, A.; Wallace, A.
    Replacing the glutamate ligand in the structural zinc site of Sulfolobus solfataricus alcohol dehydrogenase with a cysteine decreases thermostability. (1995), Protein Eng., 8, 31-37.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 expressed in Escherichia coli JM109(DE3) cells Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.1 E97C shows the same activity but a reduced thermostability with respect to the wild type recombinant protein Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 37000
-
 2 * 37000, SDS-PAGE Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1 Toyopearl Butyl 650 S column chromatography, Q Sepharose fast flow column chromatography, Superose 12 prep-grade gel filtration, and TosoHaas G 2000 SWXL gel filtration Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 0.01
-
 wild type recombinant enzyme, from crude extract, at 65°C Saccharolobus solfataricus
1.1.1.1 0.02
-
 mutant recombinant enzyme E97C, from crude extract, at 65°C Saccharolobus solfataricus
1.1.1.1 4.5
-
 wild type recombinant enzyme, after 450fold purification, at 65°C Saccharolobus solfataricus
1.1.1.1 5.2
-
 mutant recombinant enzyme E97C, after 260fold purification, at 65°C Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 benzyl alcohol + NAD+
-
 Saccharolobus solfataricus benzaldehyde + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 dimer 2 * 37000, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 SSADH
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 65
-
 the activity of the recombinant wild type enzyme slowly decreases to 50% after 16 h at 65°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 9
-
-
 Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Saccharolobus solfataricus