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Literature summary extracted from

  • Willert, E.K.; Fitzpatrick, R.; Phillips, M.A.
    Allosteric regulation of an essential trypanosome polyamine biosynthetic enzyme by a catalytically dead homolog (2007), Proc. Natl. Acad. Sci. USA, 104, 8275-8280.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.50 0.11
-
 S-adenosyl-L-methionine enzyme in complex with prozyme, absence of putrescine, 37°C, pH 8.0 Trypanosoma brucei
4.1.1.50 0.17
-
 S-adenosyl-L-methionine enzyme in complex with prozyme, presence of putrescine, 37°C, pH 8.0 Trypanosoma brucei
4.1.1.50 0.24
-
 S-adenosyl-L-methionine free enzyme, presence of putrescine, 37°C, pH 8.0 Trypanosoma brucei
4.1.1.50 0.38
-
 S-adenosyl-L-methionine free enzyme, absence of putrescine, 37°C, pH 8.0 Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.50 Trypanosoma brucei A5HNV7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.50 S-adenosyl-L-methionine
-
 Trypanosoma brucei (5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.50 More Trypanosoma brucei contains both a functional enzyme and a prozyme that has lost catalytic activity. The prozyme forms a high-affinity heterodimer with the enzyme that stimulates its activity up to 1200fold, the heterodimer is the functional enzyme in vivo Trypanosoma brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.50 0.0013
-
 S-adenosyl-L-methionine free enzyme, absence of putrescine, 37°C, pH 8.0 Trypanosoma brucei
4.1.1.50 0.0082
-
 S-adenosyl-L-methionine free enzyme, presence of putrescine, 37°C, pH 8.0 Trypanosoma brucei
4.1.1.50 1.4
-
 S-adenosyl-L-methionine enzyme in complex with prozyme, absence of putrescine, 37°C, pH 8.0 Trypanosoma brucei
4.1.1.50 1.7
-
 S-adenosyl-L-methionine enzyme in complex with prozyme, presence of putrescine, 37°C, pH 8.0 Trypanosoma brucei