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Literature summary extracted from

  • Utsumi, Y.; Nakamura, Y.
    Structural and enzymatic characterization of the isoamylase1 homo-oligomer and the isoamylase1-isoamylase2 hetero-oligomer from rice endosperm (2006), Planta, 225, 75-87.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.68 expression of OsISA2 in Escherichia coli Oryza sativa
3.2.1.68 expression of OsISA2in Escherichia coli Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.68 0.00002
-
 amylose-extender amylopectin hetero-oligomer Oryza sativa
3.2.1.68 0.00004
-
 amylose-extender amylopectin homo-oligomer Oryza sativa
3.2.1.68 0.00012
-
 amylopectin hetero-oligomer Oryza sativa
3.2.1.68 0.00018
-
 beta-limit dextrin hetero-oligomer Oryza sativa
3.2.1.68 0.00021
-
 beta-limit dextrin homo-oligomer Oryza sativa
3.2.1.68 0.00025
-
 amylopectin homo-oligomer Oryza sativa
3.2.1.68 0.00062
-
 oyster glycogen hetero-oligomer Oryza sativa
3.2.1.68 0.00067
-
 oyster glycogen homo-oligomer Oryza sativa
3.2.1.68 0.0054
-
 phytoglycogen hetero-oligomer Oryza sativa
3.2.1.68 0.0065
-
 phytoglycogen homo-oligomer Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.68 85000
-
 homooligomer OsISA1 5 * 830000 and heterooligomer OsISA1-OsISA2 6 * 85000, HPLC gel filtration Oryza sativa
3.2.1.68 420000
-
 SDS-PAGE, homooligomer, five OsISA1 Oryza sativa
3.2.1.68 510000
-
 SDS-PAGE, heterooligomer, hetero-hexamer composed of five OsISA1 and one OsISA2 Oryza sativa
3.2.1.68 830000
-
 homooligomer OsISA1 5 * 830000 and heterooligomer OsISA1-OsISA2 6 * 85000, HPLC gel filtration Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.68 Oryza sativa
-
 L. cv. Nipponbare
-
3.2.1.68 Oryza sativa O80403 L. cv. Nipponbare
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.68 wild type enzyme by HitrapQ HP and TSKgel G3000SWXL column chromatography, recombinant OsISA2 by HitrapQ HP column chromatography Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.68 endosperm
-
 Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.68 amylopectin + H2O
-
 Oryza sativa maltose + maltooligosaccharides
-
 ?
3.2.1.68 amylose-extender amylopectin + H2O
-
 Oryza sativa maltose + maltooligosaccharides
-
 ?
3.2.1.68 beta limit dextrin + H2O from maize Oryza sativa maltose + maltooligosaccharides
-
 ?
3.2.1.68 oyster glycogen + H2O
-
 Oryza sativa ?
-
 ?
3.2.1.68 oyster gylcogen + H2O
-
 Oryza sativa maltose + maltooligosaccharides
-
 ?
3.2.1.68 phytoglycogen + H2O
-
 Oryza sativa maltose + maltooligosaccharides
-
 ?
3.2.1.68 starch + H2O
-
 Oryza sativa maltose + maltooligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.68 oligomer heterooligomer OsISA1-OsISA2 6*85000, HPLC gel filtration Oryza sativa
3.2.1.68 oligomer homooligomer OsISA1 5 * 830000 and heterooligomer OsISA1-OsISA2 6 * 85000, HPLC gel filtration Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
3.2.1.68 glycogen alpha-1,6-glucanohydrolase
-
 Oryza sativa
3.2.1.68 isoamylase
-
 Oryza sativa
3.2.1.68 OsISA1
-
 Oryza sativa
3.2.1.68 OsISA2
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.68 30
-
 homo-oligomer Oryza sativa
3.2.1.68 40
-
 hetero-oligomer Oryza sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.68 40
-
 homooligomer, complete loss of activity Oryza sativa
3.2.1.68 40 50 hetero-oligomer, loss of 50% activity Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.68 6.5 7.5
-
 Oryza sativa