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Literature summary extracted from

  • Patra, B.; Ghosh Dastidar, K.; Maitra, S.; Bhattacharyya, J.; Majumder, A.L.
    Functional identification of sll1383 from Synechocystis sp PCC 6803 as L-myo-inositol 1-phosphate phosphatase (EC 3.1.3.25): molecular cloning, expression and characterization (2007), Planta, 225, 1547-1558.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.25 expressed in Escherichia coli BL-21 (DE3) cells Synechocystis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.25 Li+
-
 Synechocystis sp.
3.1.3.25 additional information Na+, K+, NH4+ have no significant effect on the enzyme activity Synechocystis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.25 0.0034
-
 DL-myo-inositol 1-phosphate in the presence of 3 mM MgCl2 in 50 mM Tris-Cl (pH 8), at 42°C Synechocystis sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.25 Mg2+ optimal activation effect at 3 mM Synechocystis sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.25 32000
-
 SDS-PAGE, recombinant enzyme Synechocystis sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.25 Synechocystis sp.
-
 strain PCC 6803
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.25 Q-Sepharose fast flow column chromatography Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.25 DL-myo-inositol 1-phosphate + H2O
-
 Synechocystis sp. DL-myo-inositol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.25 IMPase
-
 Synechocystis sp.
3.1.3.25 L-myo-inositol 1-phosphate phosphatase
-
 Synechocystis sp.
3.1.3.25 myo-inositol 1 (or 4) monophosphatase
-
 Synechocystis sp.
3.1.3.25 sll1383
-
 Synechocystis sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.25 42
-
 optimally active at about 42°C Synechocystis sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.25 45 50 more than 30% and 50% of the maximal activity is reduced at 45°C and 50°C, respectively Synechocystis sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.25 37
-
 at 37°C IMPase activity of sll1383 shows about 45% of the activity observed at 42°C Synechocystis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.25 8
-
 in 50 mM Tris-Cl buffer Synechocystis sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.25 6 9.5 about 50% of the maximum activity is retained at pH 6.0 and pH 9.5 Synechocystis sp.

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.25 6
-
 in the presence of 3 mM MgCl2 in 50 mM Tris-Cl (pH 8), at 42°C Synechocystis sp. Li+