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Literature summary extracted from
- Cold-active winter rye glucanases with ice-binding capacity (2006), Plant Physiol., 141, 1459-1472.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.73 | expressed in Escherichia coli | Secale cereale |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.73 | Secale cereale | Q1EMA6 | cultivar Musketeer | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.73 | GSTrap FF column chromatography and HiTrap Benzamidine FF column chromatography | Secale cereale |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.73 | laminarin + H2O | - |
Secale cereale | D-glucose + ? | - |
? |  |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.73 | beta-1,3-1,4-glucanase | - |
Secale cereale |
| 3.2.1.73 | GLU-1 | - |
Secale cereale |
| 3.2.1.73 | GLU-3 | - |
Secale cereale |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.73 | -8 | 60 | - |
Secale cereale |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.73 | -4 | - |
GLU-3 retains 14%-35% activity at -4°C | Secale cereale |
| 3.2.1.73 | 64 | - |
GLU-3 is irreversibly denatured above 64°C | Secale cereale |
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Release 2023.1 (January 2023)
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