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Literature summary extracted from

  • Caparros-Martin, J.A.; Reiland, S.; Koechert, K.; Cutanda, M.C.; Culianez-Macia, F.A.
    Arabidopsis thaliana AtGppl and AtGpp2: two novel low molecular weight phosphatases involved in plant glycerol metabolism (2007), Plant Mol. Biol., 63, 505-517.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.21 expressed in Escherichia coli strain TB1 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.21 3.5
-
 DL-glycerol 3-phosphate Gpp2, in 20 mM Tris-HCl pH 7.0, 5 mM MgCl2, and 10 mM DL-glycerol-3-phosphate Arabidopsis thaliana
3.1.3.21 5.2
-
 DL-glycerol 3-phosphate Gpp1, in 20 mM Tris-HCl pH 7.0, 5 mM MgCl2, and 10 mM DL-glycerol-3-phosphate Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.21 chloroplast
-
 Arabidopsis thaliana 9507
-
3.1.3.21 cytoplasm Gpp2 Arabidopsis thaliana 5737
-
3.1.3.21 plastid Gpp1 Arabidopsis thaliana 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.21 27000
-
 SDS-PAGE, Gpp2 Arabidopsis thaliana
3.1.3.21 33000
-
 SDS-PAGE, Gpp1 Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.21 Arabidopsis thaliana
-
 ecotype Columbia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.21 amylose resin column chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.21 embryo
-
 Arabidopsis thaliana
-
3.1.3.21 leaf
-
 Arabidopsis thaliana
-
3.1.3.21 meristem
-
 Arabidopsis thaliana
-
3.1.3.21 root
-
 Arabidopsis thaliana
-
3.1.3.21 shoot
-
 Arabidopsis thaliana
-
3.1.3.21 silique
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.21 DL-glycerol 3-phosphate + H2O
-
 Arabidopsis thaliana glycerol + phosphate
-
 ?
3.1.3.21 additional information poor activity is observed on glucose-6-phosphate (less than 3%), fructose-6-phosphate (less than 12%), or 2-deoxyglucose-6-phosphate (less than 2%) compared to DL-glycerol-3-phosphate Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.21 Gpp2
-
 Arabidopsis thaliana
3.1.3.21 Gppl
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.21 7
-
-
 Arabidopsis thaliana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.21 Arabidopsis thaliana isoelectric focusing, Gpp2
-
 5.6
3.1.3.21 Arabidopsis thaliana isoelectric focusing, Gpp1
-
 7.8