Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rabot, S.; Peerless, A.C.J.; Robins, R.J.
    Tigloyl-CoA:pseudotropine acyl transferase - an enzyme of tropane alkaloid biosynthesis (1995), Phytochemistry, 39, 315-322.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.186 acetyl-CoA competitively inhibits activity with tigloyl-CoA Datura stramonium
2.3.1.186 tigloyl-CoA competitively inhibits activity with acetyl-CoA Datura stramonium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.186 0.33
-
 acetyl-CoA 30°C, pH 9 Datura stramonium
2.3.1.186 0.36
-
 pseudotropine 30°C, pH 9 Datura stramonium
2.3.1.186 1.31
-
 tigloyl-CoA 30°C, pH 9 Datura stramonium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.186 65000
-
 gel filtration Datura stramonium
2.3.1.186 66000
-
 1 * 66000, SDS-PAGE Datura stramonium

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.186 Datura stramonium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.186
-
 Datura stramonium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.186 root root culture, activity is higher during rapid growth than in stationary phase roots Datura stramonium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.186 12.6
-
-
 Datura stramonium

Storage Stability

EC Number Storage Stability Organism
2.3.1.186 -20°C, stable for at least 18 months Datura stramonium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.186 3-methylcrotonyl-CoA + pseudotropine 4% of the activity with tigloyl-CoA Datura stramonium CoA + O-(3-methylcrotonyl)pseudotropine
-
 ?
2.3.1.186 acetyl-CoA + pseudotropine 4% of the activity with tigloyl-CoA Datura stramonium CoA + O-acetylpseudotropine
-
 ?
2.3.1.186 isobutyryl-CoA + pseudotropine 10% of the activity with tigloyl-CoA Datura stramonium CoA + O-isobutyrylpseudotropine
-
 ?
2.3.1.186 isovaleryl-CoA + pseudotropine 2% of the activity with tigloyl-CoA Datura stramonium CoA + O-isovalerylpseudotropine
-
 ?
2.3.1.186 additional information tropine and norpseudotropine are not acylated Datura stramonium ?
-
 ?
2.3.1.186 n-propionyl-CoA + pseudotropine 5% of the activity with tigloyl-CoA Datura stramonium CoA + O-n-propionylpseudotropine
-
 ?
2.3.1.186 tigloyl-CoA + 4-hydroxy-1-methylpiperidine 14% of the activity with pseudotropine Datura stramonium CoA + O-tigloyl-4-hydroxy-1-methylpiperidine
-
 ?
2.3.1.186 tigloyl-CoA + pseudotropine
-
 Datura stramonium CoA + O-tigloylpseudotropine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.186 monomer 1 * 66000, SDS-PAGE Datura stramonium

Synonyms

EC Number Synonyms Comment Organism
2.3.1.186 acetyl-CoA:pseudotropine acyl transferase
-
 Datura stramonium
2.3.1.186 tigloyl-CoA:pseudotropine acyl transferase
-
 Datura stramonium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.186 9
-
 activity is highest in glycine buffer, lowerv activity in Tris and borate buffer Datura stramonium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.186 7 9.5 pH 7.0: about 35% of maximal activity, pH 10.0: about 20% of 20% of maximal activity Datura stramonium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.186 Datura stramonium chromatofocusing
-
 5.4