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Literature summary extracted from

  • Kuorelahti, S.; Jouhten, P.; Maaheimo, H.; Penttilae, M.; Richard, P.
    L-galactonate dehydratase is part of the fungal path for D-galacturonic acid catabolism (2006), Mol. Microbiol., 61, 1060-1068.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.146 the LGD1 protein is tagged with a histidine tag and produced in Saccharomyces cerevisiae. In one construct the C-terminus of the protein was tagged, in another the N-terminal end of the protein Trichoderma reesei

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.146 additional information the C-terminal tagging results in an inactive protein while the N-terminal tagging results in a protein with reduced activity. The activity found in the crude extract of the N-terminally tagged protein is about five times lower than the non-tagged protein Trichoderma reesei

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.146 EDTA 5 mM, complete loss of activity Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.146 5
-
 L-galactonate pH 7.0 Trichoderma reesei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.146 additional information divalent cation required Trichoderma reesei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.146 L-galactonate Trichoderma reesei the second enzyme of the fungal pathway for D-galacturonic acid catabolism after the D-galacturonic acid reductase. A deletion of the L-galactonate dehydratase gene in Hypocrea jecorina results in a strain with no growth on D-galacturonic acid 2-dehydro-3-deoxy-L-galactonate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.146 Trichoderma reesei Q0QWS4 Trichoderma reesei
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.146 culture condition:L-galactonate-grown cell L-galactonate dehydratase activity is present in cells grown on D-galacturonic acid but absent when other carbon sources are used for growth Trichoderma reesei
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.146 D-arabonate 50% of the activity with L-galactonate Trichoderma reesei ?
-
 ?
4.2.1.146 L-galactonate
-
 Trichoderma reesei 2-dehydro-3-deoxy-L-galactonate + H2O
-
 ?
4.2.1.146 L-galactonate the second enzyme of the fungal pathway for D-galacturonic acid catabolism after the D-galacturonic acid reductase. A deletion of the L-galactonate dehydratase gene in Hypocrea jecorina results in a strain with no growth on D-galacturonic acid Trichoderma reesei 2-dehydro-3-deoxy-L-galactonate + H2O
-
 ?
4.2.1.146 additional information no activity with D-galactonate, D-gluconate, L-gulonate or D-xylonate Trichoderma reesei ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.146 LGD1
-
 Trichoderma reesei