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Literature summary extracted from

  • Marr, A.K.; Overhage, J.; Bains, M.; Hancock, R.E.
    The Lon protease of Pseudomonas aeruginosa is induced by aminoglycosides and is involved in biofilm formation and motility (2007), Microbiology, 153, 474-482.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.53 gentamicin induces lon protease by subinhibitory concentrations Pseudomonas aeruginosa
3.4.21.53 tobramycin induces lon protease by subinhibitory concentrations Pseudomonas aeruginosa

Application

EC Number Application Comment Organism
3.4.21.53 medicine subinhibitory concentrations of antibiotics induce a set of genes, e.g. lon protease, that are likely to affect the interaction of Pseudomonas aeruginosa with host cells. lon protease is essential for biofilm formation and motility in Pseudomonas aeruginosa Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.53 ORF of the lon gene PA1803 PCR-amplified and cloned into vector TopoBlunt. HindIII-XbaI fragment containing the lon gene ligated into vector pBBR1-MCS5, transformed into Escherichia coli XL-1 Blue and subsequently into Escherichia coli S17-1 for mobilization into Pseudomonas aeruginosa 74-D9 through conjugation Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.53 additional information lon mutants show impaired motility compared to the wild-type and a defect in biofilm formation Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.53 Pseudomonas aeruginosa
-
-
-

Synonyms

EC Number Synonyms Comment Organism
3.4.21.53 lon protease
-
 Pseudomonas aeruginosa