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Literature summary extracted from

  • Halak, S.; Basta, T.; Buerger, S.; Contzen, M.; Stolz, A.
    Characterization of the genes encoding the 3-carboxy-cis,cis-muconate-lactonizing enzymes from the 4-sulfocatechol degradative pathways of Hydrogenophaga intermedia S1 and Agrobacterium radiobacter S2 (2006), Microbiology, 152, 3207-3216.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.2 expression as His-tagged protein in Escherichia coli Agrobacterium tumefaciens
5.5.1.2 expression as His-tagged protein in Escherichia coli Hydrogenophaga intermedia

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.5.1.2 additional information enzyme shows significantly reduced activities in sodium/potassium phosphate/citrate buffers Agrobacterium tumefaciens
5.5.1.2 additional information enzyme shows significantly reduced activities in sodium/potassium phosphate/citrate buffers Hydrogenophaga intermedia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.2 0.15
-
 2-carboxy-2,5-dihydro-5-oxofuran-2-acetate pH 8.0, 30°C Agrobacterium tumefaciens
5.5.1.2 0.15
-
 2-carboxy-2,5-dihydro-5-oxofuran-2-acetate pH 8.0, 30°C Hydrogenophaga intermedia
5.5.1.2 7.1
-
 3-sulfomuconate pH 8.0, 30°C Hydrogenophaga intermedia
5.5.1.2 15.3
-
 3-sulfomuconate pH 8.0, 30°C Agrobacterium tumefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.5.1.2 42000
-
 x * 42000, SDS-PAGE Agrobacterium tumefaciens
5.5.1.2 42000
-
 x * 42000, SDS-PAGE Hydrogenophaga intermedia

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.2 Agrobacterium tumefaciens
-
-
-
5.5.1.2 Agrobacterium tumefaciens S2
-
-
-
5.5.1.2 Hydrogenophaga intermedia Q2HNY9
-
-
5.5.1.2 Hydrogenophaga intermedia S1 Q2HNY9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.2 recombinant protein Agrobacterium tumefaciens
5.5.1.2 recombinant protein Hydrogenophaga intermedia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.5.1.2 0.5
-
 pH 8.0, 30°C Agrobacterium tumefaciens
5.5.1.2 0.67
-
 pH 8.0, 30°C Hydrogenophaga intermedia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.2 2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
 Agrobacterium tumefaciens cis,cis-butadiene-1,2,4-tricarboxylate
-
 ?
5.5.1.2 2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
 Agrobacterium tumefaciens S2 cis,cis-butadiene-1,2,4-tricarboxylate
-
 ?
5.5.1.2 2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
 Hydrogenophaga intermedia cis-cis-butadiene-1,2,4-tricarboxylate
-
 ?
5.5.1.2 3-sulfomuconate
-
 Agrobacterium tumefaciens 4-sulfomuconolactone + H2O
-
 ?
5.5.1.2 3-sulfomuconate
-
 Hydrogenophaga intermedia 4-sulfomuconolactone + H2O
-
 ?
5.5.1.2 3-sulfomuconate
-
 Agrobacterium tumefaciens S2 4-sulfomuconolactone + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
5.5.1.2 ? x * 42000, SDS-PAGE Agrobacterium tumefaciens
5.5.1.2 ? x * 42000, SDS-PAGE Hydrogenophaga intermedia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5.1.2 0.92
-
 2-carboxy-2,5-dihydro-5-oxofuran-2-acetate pH 8.0, 30°C Agrobacterium tumefaciens
5.5.1.2 1.22
-
 2-carboxy-2,5-dihydro-5-oxofuran-2-acetate pH 8.0, 30°C Hydrogenophaga intermedia
5.5.1.2 20.9
-
 3-sulfomuconate pH 8.0, 30°C Agrobacterium tumefaciens
5.5.1.2 42.7
-
 3-sulfomuconate pH 8.0, 30°C Hydrogenophaga intermedia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5.1.2 8
-
-
 Agrobacterium tumefaciens
5.5.1.2 8
-
-
 Hydrogenophaga intermedia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.5.1.2 5.8
-
 Histidine/HCl buffer, 50% of maximum activity Agrobacterium tumefaciens
5.5.1.2 5.8
-
 Histidine/HCl buffer, 50% of maximum activity Hydrogenophaga intermedia
5.5.1.2 9
-
 Tris/HCl buffer, 50% of maximum activity Agrobacterium tumefaciens
5.5.1.2 9
-
 Tris/HCl buffer, 50% of maximum activity Hydrogenophaga intermedia