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Literature summary extracted from

  • Li, X.; Chi, Z.; Liu, Z.; Li, J.; Wang, X.; Hirimuthugoda, N.Y.
    Purification and characterization of extracellular phytase from a marine yeast Kodamaea ohmeri BG3 (2008), Mar. Biotechnol., 10, 190-197.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 Ag+ 49% inhibitory effect at 5 mM Kodamaea ohmeri
3.1.3.8 Cu2+ 17.5% inhibitory effect at 5 mM Kodamaea ohmeri
3.1.3.8 Fe2+ 25.7% inhibitory effect at 5 mM Kodamaea ohmeri
3.1.3.8 Fe3+ 19.4% inhibitory effect at 5 mM Kodamaea ohmeri
3.1.3.8 Hg2+ 99.2% inhibitory effect at 5 mM Kodamaea ohmeri
3.1.3.8 iodoacetic acid 6.3% inhibitory effect at 1 mM Kodamaea ohmeri
3.1.3.8 additional information not inhibited by 1 mM EDTA and 5 mM 1,10-phenanthroline Kodamaea ohmeri
3.1.3.8 phenylglyoxal hydrate 45.3% inhibitory effect at 5 mM Kodamaea ohmeri
3.1.3.8 phenylmethylsulfonyl fluoride 11.8% inhibitory effect at 10 mM Kodamaea ohmeri
3.1.3.8 Zn2+ 30.3% inhibitory effect at 5 mM Kodamaea ohmeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 1.45
-
 myo-inositol hexakisphosphate 5.0 mM myo-inositol hexakisphosphate in 0.2 M sodium acetate, pH 5.0, at 60°C Kodamaea ohmeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.8 Ba2+ 19.5% stimulatory effect at 5 mM Kodamaea ohmeri
3.1.3.8 Ca2+ 10.8% stimulatory effect at 5 mM Kodamaea ohmeri
3.1.3.8 Co2+ 25.5% stimulatory effect at 5 mM Kodamaea ohmeri
3.1.3.8 K+ 14.9% stimulatory effect at 5 mM Kodamaea ohmeri
3.1.3.8 Li+ 17.5% stimulatory effect at 5 mM Kodamaea ohmeri
3.1.3.8 Mg2+ 21.7% stimulatory effect at 5 mM Kodamaea ohmeri
3.1.3.8 Mn2+ 17.8% stimulatory effect at 5 mM Kodamaea ohmeri
3.1.3.8 Na+ 14.8% stimulatory effect at 5 mM Kodamaea ohmeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 92900
-
 gel filtration Kodamaea ohmeri
3.1.3.8 98200
-
 SDS-PAGE Kodamaea ohmeri

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Kodamaea ohmeri
-
-
-
3.1.3.8 Kodamaea ohmeri BG3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 ammonium sulfate fractionation, Sephadex G-75 gel filtration, and DEAE Sepharose Fast Flow column chromatography Kodamaea ohmeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 114.6
-
 supernantant, at 60°C Kodamaea ohmeri
3.1.3.8 823.3
-
 after 7.2fold purification, at 60°C Kodamaea ohmeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Kodamaea ohmeri 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Kodamaea ohmeri BG3 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 monomer 1 * 92900, gel filtration Kodamaea ohmeri

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 PhyA
-
 Kodamaea ohmeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 65
-
-
 Kodamaea ohmeri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 60 70 the residual phytase activity still maintains 96.3% of the control after treatment at 60°C for 1 h, the enzyme is inactivated rapidly at temperatures higher than 60°C and is almost inactivated at 70°C within 1 h Kodamaea ohmeri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 0.93
-
 myo-inositol hexakisphosphate 5.0 mM myo-inositol hexakisphosphate in 0.2 M sodium acetate, pH 5.0, at 60°C Kodamaea ohmeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5
-
-
 Kodamaea ohmeri

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.8 3 9 more than 94.0% of the residual activity is maintained after treatment at pH from 3.0 to 9.0 Kodamaea ohmeri