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Literature summary extracted from

  • Zhao, D.M.; Wang, M.; Mu, X.J.; Sun, M.L.; Wang, X.Y.
    Screening, cloning and overexpression of Aspergillus niger phytase (phyA) in Pichia pastoris with favourable characteristics (2007), Lett. Appl. Microbiol., 45, 522-528.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 expressed in Pichia pastoris strain GS115 Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.196
-
 myo-inositol hexakisphosphate in 1 M sodium acetate buffer pH 5.5, at 37°C Aspergillus niger
3.1.3.8 18.16
-
 p-nitrophenyl phosphate in 1 M sodium acetate buffer pH 5.5, at 37°C Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 49200
-
 calculated molecular weight Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus niger Q6T9Z6
-
-
3.1.3.8 Aspergillus niger N-J Q6T9Z6
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 503
-
 recombinant enzyme, at 37°C Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Aspergillus niger 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Aspergillus niger N-J 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
3.1.3.8 p-nitrophenyl phosphate + H2O
-
 Aspergillus niger p-nitrophenol + phosphate
-
 ?
3.1.3.8 p-nitrophenyl phosphate + H2O
-
 Aspergillus niger N-J p-nitrophenol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 myo-inositol hexaphosphate phosphohydrolase
-
 Aspergillus niger
3.1.3.8 PhyA
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 55
-
 recombinant enzyme, with 10 min reaction time Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 90
-
 retains 70% activity after exposure to 90°C for 5 min and 65% activity after 30 min Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 1.9
-
 p-nitrophenyl phosphate in 1 M sodium acetate buffer pH 5.5, at 37°C Aspergillus niger
3.1.3.8 398
-
 myo-inositol hexakisphosphate in 1 M sodium acetate buffer pH 5.5, at 37°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 2.5
-
 recombinant enzyme Aspergillus niger
3.1.3.8 5.5
-
 recombinant enzyme Aspergillus niger