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Literature summary extracted from
- Dehydroepiandrosterone sulphate sulphohydrolase from human placenta microsomes - properties of the purified enzyme (2006), J. Steroid Biochem. Mol. Biol., 99, 67-75.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.6.2 | glutathione | oxidized form, 5 mM, 187% of initial activity | Homo sapiens |  |
| 3.1.6.2 | L-beta-phenylalanine | 5 mM, 169% of initial activity | Homo sapiens | |
| 3.1.6.2 | L-cysteine | 5 mM, 202% of initial activity | Homo sapiens | |
| 3.1.6.2 | lithocholic acid | 5 mM, 226% of initial activity | Homo sapiens | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.6.2 | Ammonium molybdate | 10 mM, 20% residual activity | Homo sapiens | |
| 3.1.6.2 | calcium chloride | 5 mM, 36% residual activity | Homo sapiens | |
| 3.1.6.2 | dithiothreitol | 5 mM, 45% residual activity | Homo sapiens | |
| 3.1.6.2 | glutathione | reduced form, 5 mM, 23.5% residual activity | Homo sapiens | |
| 3.1.6.2 | additional information | not inhibitory: phosphate, sulfate, sulfide | Homo sapiens | |
| 3.1.6.2 | vanadium oxide(V) | 50 mM, 51% residual activity | Homo sapiens | |
| 3.1.6.2 | Zinc acetate | 1.25 mM, 53% residual activity | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.6.2 | 0.033 | - |
dehydroepiandrosterone 3-sulfate | - |
Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.6.2 | microsome | - |
Homo sapiens | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.2 | 62000 | - |
x * 62000, SDS-PAGE | Homo sapiens |
| 3.1.6.2 | 74000 | - |
gel filtration | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.6.2 | Homo sapiens | - |
enzyme does not copurify with oestrone sulfate sulfohydrolase | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.6.2 | enzyme does not copurify with oestrone sulfate sulfohydrolase | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.6.2 | placenta | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.2 | 1.52 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.6.2 | dehydroepiandrosterone 3-sulfate + H2O | - |
Homo sapiens | dehydroepiandrosterone + sulfate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.6.2 | ? | x * 62000, SDS-PAGE | Homo sapiens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.2 | 60 | - |
5 min, 115% resiudal activity | Homo sapiens |
| 3.1.6.2 | 80 | - |
5 min, 100% residual activity | Homo sapiens |
| 3.1.6.2 | 100 | - |
5 min, 87% residual activity | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.2 | 8.4 | - |
- |
Homo sapiens |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.6.2 | Homo sapiens | isoelectric focusing | - |
8.7 |
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