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Literature summary extracted from

  • Hwang, S.R.; ONeill, A.; Bark, S.; Foulon, T.; Hook, V.
    Secretory vesicle aminopeptidase B related to neuropeptide processing: molecular identification and subcellular localization to enkephalin- and NPY-containing chromaffin granules (2007), J. Neurochem., 100, 1340-1350.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.6 expressed in Escherichia coli Rattus norvegicus
3.4.11.6 expressed in Escherichia coli Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.6 arphamenine A complete inhibition at 0.1 mM Bos taurus
3.4.11.6 arphamenine A complete inhibition at 0.1 mM Rattus norvegicus
3.4.11.6 bestatin complete inhibition at 0.1 mM Bos taurus
3.4.11.6 bestatin complete inhibition at 0.1 mM Rattus norvegicus
3.4.11.6 beta-mercaptoethanol 4% inhibition at 10 mM Bos taurus
3.4.11.6 beta-mercaptoethanol 20% inhibition at 10 mM Rattus norvegicus
3.4.11.6 Co2+ 62% inhibition at 1 mM Bos taurus
3.4.11.6 Co2+ 45% inhibition at 1 mM Rattus norvegicus
3.4.11.6 cysteine 21% inhibition at 1 mM Bos taurus
3.4.11.6 cysteine 15% inhibition at 1 mM Rattus norvegicus
3.4.11.6 dithiothreitol 33% inhibition at 10 mM Bos taurus
3.4.11.6 dithiothreitol 54% inhibition at 10 mM Rattus norvegicus
3.4.11.6 E64 48% inhibition at 0.01 mM Bos taurus
3.4.11.6 E64 12% inhibition at 0.01 mM Rattus norvegicus
3.4.11.6 EDTA 12% inhibition at 1 mM Bos taurus
3.4.11.6 EDTA 54% inhibition at 1 mM Rattus norvegicus
3.4.11.6 N-ethylmaleimide 44% inhibition at 0.1 mM Bos taurus
3.4.11.6 N-ethylmaleimide 21% inhibition at 0.1 mM Rattus norvegicus
3.4.11.6 Ni2+ 75% inhibition at 1 mM Bos taurus
3.4.11.6 Ni2+ 63% inhibition at 1 mM Rattus norvegicus
3.4.11.6 Phenylmethanesulfonylfluoride 10% inhibition at 0.1 mM Bos taurus
3.4.11.6 Phenylmethanesulfonylfluoride 1% inhibition at 0.1 mM Rattus norvegicus
3.4.11.6 puromycin 10% inhibition at 0.1 mM Bos taurus
3.4.11.6 puromycin 1% inhibition at 0.1 mM Rattus norvegicus
3.4.11.6 Zn2+ complete inhibition at 0.1 mM Bos taurus
3.4.11.6 Zn2+ 99% inhibition at 0.1 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.6 0.048
-
 L-Arg-7-amido-4-methylcoumarin
-
 Rattus norvegicus
3.4.11.6 0.091
-
 L-Arg-7-amido-4-methylcoumarin
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.6 secretory vesicle present in secretory vesicles that contain cathepsin L with the neuropeptides enkephalin and neuropeptide Y Rattus norvegicus 99503
-
3.4.11.6 secretory vesicle present in secretory vesicles that contain cathepsin L with the neuropeptides enkephalin and neuropeptide Y Bos taurus 99503
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.6 Na+ 110% increase of activity at 150 mM Rattus norvegicus
3.4.11.6 Na+ 380% increase of activity at 150 mM Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.6 72100
-
 calculated from amino acid sequence Bos taurus
3.4.11.6 74000
-
 SDS-PAGE Rattus norvegicus
3.4.11.6 74000
-
 SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.6 Bos taurus A2T1U6
-
-
3.4.11.6 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.6
-
 Rattus norvegicus
3.4.11.6
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.6 adrenal medulla
-
 Rattus norvegicus
-
3.4.11.6 adrenal medulla
-
 Bos taurus
-
3.4.11.6 brain
-
 Rattus norvegicus
-
3.4.11.6 hypothalamus
-
 Rattus norvegicus
-
3.4.11.6 pancreas
-
 Rattus norvegicus
-
3.4.11.6 pituitary gland
-
 Rattus norvegicus
-
3.4.11.6 pituitary gland
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.6 L-Arg-(Met)enkephalin + H2O
-
 Rattus norvegicus L-Arg + (Met)enkephalin
-
 ?
3.4.11.6 L-Arg-(Met)enkephalin + H2O
-
 Bos taurus L-Arg + (Met)enkephalin
-
 ?
3.4.11.6 L-Arg-7-amido-4-methylcoumarin + H2O 100% activity Rattus norvegicus L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Arg-7-amido-4-methylcoumarin + H2O 100% activity Bos taurus L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Asn-7-amido-4-methylcoumarin + H2O 53% activity compared to L-Arg-7-amido-4-methylcoumarin Bos taurus L-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Asp-7-amido-4-methylcoumarin + H2O 15% activity compared to L-Arg-7-amido-4-methylcoumarin Bos taurus L-Asp + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Cys-7-amido-4-methylcoumarin + H2O 5% activity compared to L-Arg-7-amido-4-methylcoumarin Rattus norvegicus L-Cys + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Leu-7-amido-4-methylcoumarin + H2O 28% activity compared to L-Arg-7-amido-4-methylcoumarin Bos taurus L-Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Lys-(Met)enkephalin + H2O
-
 Rattus norvegicus L-Lys + (Met)enkephalin
-
 ?
3.4.11.6 L-Lys-(Met)enkephalin + H2O
-
 Bos taurus L-Lys + (Met)enkephalin
-
 ?
3.4.11.6 L-Lys-7-amido-4-methylcoumarin + H2O 10% activity compared to L-Arg-7-amido-4-methylcoumarin Bos taurus L-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Lys-7-amido-4-methylcoumarin + H2O 17% activity compared to L-Arg-7-amido-4-methylcoumarin Rattus norvegicus L-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Met-7-amido-4-methylcoumarin + H2O 19% activity compared to L-Arg-7-amido-4-methylcoumarin Bos taurus L-Met + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Phe-7-amido-4-methylcoumarin + H2O 2% activity compared to L-Arg-7-amido-4-methylcoumarin Rattus norvegicus L-Phe + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Pro-7-amido-4-methylcoumarin + H2O 7% activity compared to L-Arg-7-amido-4-methylcoumarin Rattus norvegicus L-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Ser-7-amido-4-methylcoumarin + H2O 15% activity compared to L-Arg-7-amido-4-methylcoumarin Bos taurus L-Ser + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 additional information no activity with L-Ala-7-amido-4-methylcoumarin, L-Asn-7-amido-4-methylcoumarin, L-Gln-7-amido-4-methylcoumarin, L-Glu-7-amido-4-methylcoumarin, Gly-7-amido-4-methylcoumarin, L-His-7-amido-4-methylcoumarin, L-Ile-7-amido-4-methylcoumarin, L-Leu-7-amido-4-methylcoumarin, L-Asp-7-amido-4-methylcoumarin, L-Thr-7-amido-4-methylcoumarin, L-Met-7-amido-4-methylcoumarin, L-Trp-7-amido-4-methylcoumarin, L-Ser-7-amido-4-methylcoumarin, L-Tyr-7-amido-4-methylcoumarin, and L-Val-7-amido-4-methylcoumarin Rattus norvegicus ?
-
 ?
3.4.11.6 additional information no activity with L-Ala-7-amido-4-methylcoumarin, L-Cys-7-amido-4-methylcoumarin, L-Gln-7-amido-4-methylcoumarin, L-Glu-7-amido-4-methylcoumarin, Gly-7-amido-4-methylcoumarin, L-His-7-amido-4-methylcoumarin, L-Ile-7-amido-4-methylcoumarin, L-Phe-7-amido-4-methylcoumarin, L-Pro-7-amido-4-methylcoumarin, L-Thr-7-amido-4-methylcoumarin, L-Trp-7-amido-4-methylcoumarin, L-Tyr-7-amido-4-methylcoumarin, and L-Val-7-amido-4-methylcoumarin Bos taurus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.6 Ap-B
-
 Rattus norvegicus
3.4.11.6 Ap-B
-
 Bos taurus
3.4.11.6 Arg/Lys aminopeptidase
-
 Rattus norvegicus
3.4.11.6 Arg/Lys aminopeptidase
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.6 5.5 6.5
-
 Rattus norvegicus
3.4.11.6 5.5 6.5
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.6 5 7
-
 Rattus norvegicus
3.4.11.6 5 7
-
 Bos taurus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.6 Bos taurus calculated from amino acid sequence
-
 5.3