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BRENDA support

Literature summary extracted from

  • Gupta, P.; Maqbool, T.; Saleemuddin, M.
    Oriented immobilization of stem bromelain via the lone histidine on a metal affinity support (2007), J. Mol. Catal. B, 45, 78-83.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.4.22.32 bromelain immobilized to IDA-Sepharose 6B matrix loaded with Cu2+, Ni2+ and Zn2+ is more resistant to thermal inactivation, as evidenced by retention of over 50% activity after incubation at 60°C Ananas comosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.32 23800
-
 SDS-PAGE Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.32 Ananas comosus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.32 stem
-
 Ananas comosus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.32 casein + H2O
-
 Ananas comosus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.32 Bromelain
-
 Ananas comosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.32 60
-
 the native enzyme retains 20% of the initial activity after incubation at 60°C Ananas comosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.32 6
-
 immobilized enzyme Ananas comosus
3.4.22.32 7.5
-
 native enzyme Ananas comosus