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Literature summary extracted from

  • Vardakou, M.; Dumon, C.; Murray, J.W.; Christakopoulos, P.; Weiner, D.P.; Juge, N.; Lewis, R.J.; Gilbert, H.J.; Flint, J.E.
    Understanding the structural basis for substrate and inhibitor recognition in eukaryotic GH11 xylanases (2008), J. Mol. Biol., 375, 1293-1305.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 expressed in Escherichia coli Neocallimastix patriciarum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.8 enzyme bound to ferulic acid-1,5-arabinofuranose-alpha-1,3-xylotriose, hanging drop vapour diffusion method, with 0.1 M cadmium chloride, 0.1 M sodium acetate (pH 4.6) and 30% (w/v) polyethylene glycol 400 Neocallimastix patriciarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 additional information not inhibited by XIP-I Neocallimastix patriciarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 2.1
-
 4-nitrophenyl-beta-D-xylotrioside in 12 mM sodium citrate, 50 mM sodium phosphate buffer (pH 6.5) Neocallimastix patriciarum
3.2.1.8 15
-
 4-nitrophenyl-beta-D-xylobioside in 12 mM sodium citrate, 50 mM sodium phosphate buffer (pH 6.5) Neocallimastix patriciarum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Neocallimastix patriciarum P29127 bifunctional endo-1,4-beta-xylanase A precursor
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 Q10 anion exchange column chromatography Neocallimastix patriciarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 4-nitrophenyl beta-D-xylobioside + H2O
-
 Neocallimastix patriciarum ?
-
 ?
3.2.1.8 4-nitrophenyl beta-D-xylotrioside + H2O
-
 Neocallimastix patriciarum ?
-
 ?
3.2.1.8 oat spelt xylan + H2O
-
 Neocallimastix patriciarum ?
-
 ?
3.2.1.8 xylohexaose + H2O
-
 Neocallimastix patriciarum ?
-
 ?
3.2.1.8 xylopentaose + H2O
-
 Neocallimastix patriciarum ?
-
 ?
3.2.1.8 xylotetraose + H2O
-
 Neocallimastix patriciarum ?
-
 ?
3.2.1.8 xylotriose + H2O
-
 Neocallimastix patriciarum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 GH11 xylanase
-
 Neocallimastix patriciarum
3.2.1.8 xylanase
-
 Neocallimastix patriciarum
3.2.1.8 Xyn11A
-
 Neocallimastix patriciarum