EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.15.1.1 | purified native and recombinant enzyme, hanging drop vapour diffusion method, 21°C, 2 mg/ml protein, from 8% v/v PEG 8000, 0.1 M Tris-HCl, pH 8.5, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement | Saccharolobus solfataricus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.15.1.1 | Fe2+ | Fe-type SOD, helices alpha1 and alpha2 contribute one metal ligand each, i.e. His33 and His84, binding structure, the iron is ligated by Nepsilon2 of His33, His84 and His174, by Odelta1 of Asp170, and a solvent molecule forming a distorted trigonal bipyramidal coordination sphere, overview | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | 24000 | - |
4 * 24000, alpha2beta2, SDS-PAGE | Saccharolobus solfataricus |
1.15.1.1 | 87900 | - |
gel filtration | Saccharolobus solfataricus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.15.1.1 | 2 O2.- + 2 H+ | Saccharolobus solfataricus | superoxide dismutase is a key enzyme for the protection of aerobic organisms against toxic radicals produced during oxidative processes | O2 + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.15.1.1 | Saccharolobus solfataricus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.15.1.1 | 2 O2.- + 2 H+ | superoxide dismutase is a key enzyme for the protection of aerobic organisms against toxic radicals produced during oxidative processes | Saccharolobus solfataricus | O2 + H2O2 | - |
? | |
1.15.1.1 | 2 O2.- + 2 H+ | dismutation of superoxide in a two-step reaction: 1. O2.- + Fe3+-SOD = O2 + Fe2+-SOD, 2. O2.- + Fe2+-SOD + 2 H+ = H2O2 + Fe3+-SOD | Saccharolobus solfataricus | O2 + H2O2 | - |
? | |
1.15.1.1 | additional information | unusual covalent modification of the conserved Tyr41 in the active site, interactions Tyr41-His155, overview | Saccharolobus solfataricus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.15.1.1 | tetramer | 4 * 24000, alpha2beta2, SDS-PAGE | Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.15.1.1 | SOD | - |
Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | 28 | - |
assay at | Saccharolobus solfataricus |