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Literature summary extracted from

  • Yi, Y.; Qiao, D.; Bai, L.; Xu, H.; Li, Y.; Wang, X.; Cao, Y.
    Cloning, expression, and functional characterization of the Dunaliella salina 5-enolpyruvylshikimate-3-phosphate synthase gene in Escherichia coli (2007), J. Microbiol., 45, 153-157.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.19 biotechnology glyphosate tolerance in bacteria Dunaliella salina

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.19 amplified by 3’ and 5’ RACE on total RNA according to an EST found in Dunaliella salina cDNA library, cloned into pMD18-T for sequencing, cloned in pGEX-4T-1 for expression as mature protein (lacking N-terminal 70 amino acids long transit peptide) in Escherichia coli JM109 or EPSP synthase gene deleted Escherichia coli strain ER2799 Dunaliella salina
2.5.1.19 expression in Escherichia coli Dunaliella salina

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.19 chloroplast putative N-terminal transit peptide consisting of 70 amino acids which is present in other plant EPSP synthases and lacking in the bacterial enzyme Dunaliella salina 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.19 54600
-
 deduced from primary structure, 514 amino acids Dunaliella salina
2.5.1.19 73000
-
 overexpressed mature protein (47000 Da) with GST tag (26000 Da) Dunaliella salina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.19 additional information Dunaliella salina sixth enzyme in shikimate pathway, biosynthesis of aromatic amino acids ?
-
 ?
2.5.1.19 additional information Dunaliella salina 435 sixth enzyme in shikimate pathway, biosynthesis of aromatic amino acids ?
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Dunaliella salina sixth enzyme in the shikimate pathway. The enzyme is widely involved in glyphosate tolerant transgenic plants because it is the primary target of the nonselective herbicide lyphosate phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Dunaliella salina 435 sixth enzyme in the shikimate pathway. The enzyme is widely involved in glyphosate tolerant transgenic plants because it is the primary target of the nonselective herbicide lyphosate phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Dunaliella salina A2T9R8
-
-
2.5.1.19 Dunaliella salina 435 A2T9R8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 additional information sixth enzyme in shikimate pathway, biosynthesis of aromatic amino acids Dunaliella salina ?
-
 ?
2.5.1.19 additional information sixth enzyme in shikimate pathway, biosynthesis of aromatic amino acids Dunaliella salina 435 ?
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate sixth enzyme in the shikimate pathway. The enzyme is widely involved in glyphosate tolerant transgenic plants because it is the primary target of the nonselective herbicide lyphosate Dunaliella salina phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate sixth enzyme in the shikimate pathway. The enzyme is widely involved in glyphosate tolerant transgenic plants because it is the primary target of the nonselective herbicide lyphosate Dunaliella salina 435 phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?
2.5.1.19 shikimate 3-phosphate + phosphoenolpyruvate activity independent of transit peptide (N-terminal 70 residues) Dunaliella salina phosphate + 5-enpolpyruvylshikimate 3-phosphate growth of transformed Escherichia coli lacking endogenous EPSP synthase on M9 minimal medium by sustaining synthesis of aromatic amino acids through introduced 5-enolpyruvylshikimate 3-phosphate synthesis, leading to 10fold increase in glyphosate tolerance in bacteria compared to controls ?
2.5.1.19 shikimate 3-phosphate + phosphoenolpyruvate activity independent of transit peptide (N-terminal 70 residues) Dunaliella salina 435 phosphate + 5-enpolpyruvylshikimate 3-phosphate growth of transformed Escherichia coli lacking endogenous EPSP synthase on M9 minimal medium by sustaining synthesis of aromatic amino acids through introduced 5-enolpyruvylshikimate 3-phosphate synthesis, leading to 10fold increase in glyphosate tolerance in bacteria compared to controls ?

Subunits

EC Number Subunits Comment Organism
2.5.1.19 ? x * 54600, cylculated from sequence Dunaliella salina

Synonyms

EC Number Synonyms Comment Organism
2.5.1.19 DsaroA
-
 Dunaliella salina
2.5.1.19 Dunaliella salina 5-enolpyruvylshikimate-3-phosphate synthase
-
 Dunaliella salina
2.5.1.19 Dunaliella salina EPSP synthase
-
 Dunaliella salina