EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.28 | 0.44 | - |
trehalose | soluble trehalase P II, inacetate buffer, pH 5.5, at 30°C | Bombyx mori | |
3.2.1.28 | 1.56 | - |
trehalose | soluble trehalase P I, in acetate buffer, pH 5.5, at 30°C | Bombyx mori |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 73000 | - |
soluble trehalase P I, SDS-PAGE | Bombyx mori |
3.2.1.28 | 140000 | - |
soluble trehalase P II, SDS-PAGE | Bombyx mori |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.28 | Bombyx mori | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.28 | Sephacryl S-300 gel filtration, DEAE-cellulofine column chromatography, and Con A-Sepharose column chromatography | Bombyx mori |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.28 | egg | - |
Bombyx mori | - |
3.2.1.28 | embryo | - |
Bombyx mori | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 0.01 | - |
soluble trehalase P I from supernatant, in acetate buffer, pH 5.5, at 30°C | Bombyx mori |
3.2.1.28 | 0.01 | - |
soluble trehalase P II from supernatant, in acetate buffer, pH 5.5, at 30°C | Bombyx mori |
3.2.1.28 | 1.03 | - |
soluble trehalase P I after 86.9fold purification, in acetate buffer, pH 5.5, at 30°C | Bombyx mori |
3.2.1.28 | 2.9 | - |
soluble trehalase P II after 245.7fold purification, in acetate buffer, pH 5.5, at 30°C | Bombyx mori |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.28 | trehalose + H2O | - |
Bombyx mori | D-glucose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.28 | acid trehalase | - |
Bombyx mori |
3.2.1.28 | alpha,alpha glucoside 1-glucohydrolase | - |
Bombyx mori |
3.2.1.28 | soluble P I type trehalase | - |
Bombyx mori |
3.2.1.28 | soluble P II type trehalase | - |
Bombyx mori |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 40 | 45 | soluble trehalase P I | Bombyx mori |
3.2.1.28 | 50 | - |
above 50°C, soluble trehalase P I | Bombyx mori |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 5.5 | - |
soluble trehalase P II | Bombyx mori |
3.2.1.28 | 6.5 | - |
soluble trehalase P I | Bombyx mori |