Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Colombres, M.; Garate, J.A.; Lagos, C.F.; Araya-Secchi, R.; Norambuena, P.; Quiroz, S.; Larrondo, L.; Perez-Acle, T.; Eyzaguirre, J.
    An eleven amino acid residue deletion expands the substrate specificity of acetyl xylan esterase II (AXE II) from Penicillium purpurogenum (2008), J. Comput. Aided Mol. Des., 22, 19-28.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.72 native and mutant protein is expressed in Aspergillus nidulans A722 Talaromyces purpureogenus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.72 structure is determined by X-ray crystallography at 0.9 resolution Talaromyces purpureogenus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.72 additional information preparation of a deletion mutant where the amino acid 104-114 are eliminated. This mutant is catalytically active and can hydrolyze long chain fatty acid esters with much greater efficiency than the wild type AXE II Talaromyces purpureogenus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.72 21900
-
 x * 21900, mutant enzyme, SDS-PAGE Talaromyces purpureogenus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.72 Talaromyces purpureogenus O59893 AXE2 precursor
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.72
-
 Talaromyces purpureogenus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.72 culture medium
-
 Talaromyces purpureogenus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.72 1-naphthyl acetate + H2O
-
 Talaromyces purpureogenus 1-naphthol + acetate
-
 ?
3.1.1.72 1-naphthyl caprate + H2O
-
 Talaromyces purpureogenus 1-naphthol + caprate
-
 ?
3.1.1.72 1-naphthyl myristate + H2O
-
 Talaromyces purpureogenus 1-naphthol + myristate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.72 ? x * 21900, mutant enzyme, SDS-PAGE Talaromyces purpureogenus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.72 acetyl xylan esterase II
-
 Talaromyces purpureogenus
3.1.1.72 AXE II
-
 Talaromyces purpureogenus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.72 60
-
 mutant enzyme Talaromyces purpureogenus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.72 70
-
 mutant enzyme is less stable than the native recombinant protein at 70°C Talaromyces purpureogenus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.72 Talaromyces purpureogenus mutant enzyme, isoelectric focusing
-
 5.9
3.1.1.72 Talaromyces purpureogenus recombinant native enzyme
-
 6.8