Literature summary extracted from

Sharma, V.; Gupta, R.; Jhingran, A.; Singh, B.P.; Sridhara, S.; Gaur, S.N.; Arora, N.
Cloning, recombinant expression and activity studies of a major allergen 'enolase' from the fungus Curvularia lunata (2006), J. Clin. Immunol., 26, 360-369.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.2.1.11	isolated from cDNA library, expressed in Escherichia coli BL21, expression vector pET22b, 60% sequence similarities to human non-neuronal alpha enolase, conserved residues determined	Curvularia lunata

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
4.2.1.11	Mg2+	metal-binding	Curvularia lunata

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
4.2.1.11	48000	-	SDS-PAGE	Curvularia lunata

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4.2.1.11	2-phospho-D-glycerate	Curvularia lunata	-	phosphoenolpyruvate + H2O	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.2.1.11	Curvularia lunata	Q96VP4	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.2.1.11	recombinant protein amounts are 10-15% of the total protein, purification from inclusion bodies yields 0.5 mg per liter, allergenic activity of the recombinant protein tested by ELIZA	Curvularia lunata

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
4.2.1.11	culture medium	4 d old	Curvularia lunata	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.2.1.11	additional information	-	biological activity shown, enzyme activity comparable to those of Candida	Curvularia lunata

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.2.1.11	2-phospho-D-glycerate	-	Curvularia lunata	phosphoenolpyruvate + H2O	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
4.2.1.11	enolase	-	Curvularia lunata

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Release 2023.1 (January 2023)