BRENDA - Enzyme Database

Cloning, recombinant expression and activity studies of a major allergen 'enolase' from the fungus Curvularia lunata

Sharma, V.; Gupta, R.; Jhingran, A.; Singh, B.P.; Sridhara, S.; Gaur, S.N.; Arora, N.; J. Clin. Immunol. 26, 360-369 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
isolated from cDNA library, expressed in Escherichia coli BL21, expression vector pET22b, 60% sequence similarities to human non-neuronal alpha enolase, conserved residues determined
Curvularia lunata
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
metal-binding
Curvularia lunata
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
48000
-
SDS-PAGE
Curvularia lunata
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Curvularia lunata
-
phosphoenolpyruvate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Curvularia lunata
Q96VP4
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
recombinant protein amounts are 10-15% of the total protein, purification from inclusion bodies yields 0.5 mg per liter, allergenic activity of the recombinant protein tested by ELIZA
Curvularia lunata
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
culture medium
4 d old
Curvularia lunata
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
biological activity shown, enzyme activity comparable to those of Candida
Curvularia lunata
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
681072
Curvularia lunata
phosphoenolpyruvate + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
isolated from cDNA library, expressed in Escherichia coli BL21, expression vector pET22b, 60% sequence similarities to human non-neuronal alpha enolase, conserved residues determined
Curvularia lunata
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
metal-binding
Curvularia lunata
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
48000
-
SDS-PAGE
Curvularia lunata
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Curvularia lunata
-
phosphoenolpyruvate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
recombinant protein amounts are 10-15% of the total protein, purification from inclusion bodies yields 0.5 mg per liter, allergenic activity of the recombinant protein tested by ELIZA
Curvularia lunata
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
culture medium
4 d old
Curvularia lunata
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
biological activity shown, enzyme activity comparable to those of Candida
Curvularia lunata
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
681072
Curvularia lunata
phosphoenolpyruvate + H2O
-
-
-
?